TAXONOMY

Nature Subjects Taxonomy

CONCEPT NAME

Two-hybrid system

DESCRIPTION

Two-hybrid systems are methods that assay for the interaction between two proteins, or protein and DNA in cells. Physical interaction is detected by the reconstitution of complementary domains of a reporter or transcription factor that activates reporter gene expression.

Related objects

BROADER TERMS

  • Cytological Techniques

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    69 TRIPLES      13 PREDICATES      69 URIs      60 LITERALS

    Subject Predicate Object
    1 subjects:two-hybrid-system dcterms:modified 2017-10-09T07:39:58+00:00
    2 sg:license http://scigraph.springernature.com/explorer/license/
    3 rdf:type npg:Subject
    4 sg:Subject
    5 skos:Concept
    6 rdfs:label Two-hybrid system
    7 skos:altLabel One Hybrid System Technics
    8 One Hybrid System Techniques
    9 One-Hybrid System Technic
    10 One-Hybrid System Technics
    11 One-Hybrid System Technique
    12 One-Hybrid System Techniques
    13 Reverse One Hybrid System Techniques
    14 Reverse One-Hybrid System Techniques
    15 Reverse Three Hybrid System Techniques
    16 Reverse Three-Hybrid System Techniques
    17 Reverse Two Hybrid System Techniques
    18 Reverse Two-Hybrid System Techniques
    19 Three Hybrid System Technics
    20 Three Hybrid System Techniques
    21 Three-Hybrid System Technic
    22 Three-Hybrid System Technics
    23 Three-Hybrid System Technique
    24 Three-Hybrid System Techniques
    25 Tri Hybrid System Techniques
    26 Tri-Hybrid System Technique
    27 Tri-Hybrid System Techniques
    28 Two Hybrid Assay
    29 Two Hybrid Method
    30 Two Hybrid System Technics
    31 Two Hybrid System Techniques
    32 Two-Hybrid Assay
    33 Two-Hybrid Assays
    34 Two-Hybrid Method
    35 Two-Hybrid Methods
    36 Two-Hybrid System Technic
    37 Two-Hybrid System Technics
    38 Two-Hybrid System Technique
    39 Two-Hybrid System Techniques
    40 Yeast One Hybrid System Techniques
    41 Yeast One-Hybrid System Techniques
    42 Yeast Three Hybrid Assay
    43 Yeast Three Hybrid System
    44 Yeast Three Hybrid System Techniques
    45 Yeast Three-Hybrid Assay
    46 Yeast Three-Hybrid Assays
    47 Yeast Three-Hybrid System
    48 Yeast Three-Hybrid System Techniques
    49 Yeast Three-Hybrid Systems
    50 Yeast Two Hybrid Assay
    51 Yeast Two Hybrid System
    52 Yeast Two Hybrid System Techniques
    53 Yeast Two-Hybrid Assay
    54 Yeast Two-Hybrid Assays
    55 Yeast Two-Hybrid System
    56 Yeast Two-Hybrid System Techniques
    57 Yeast Two-Hybrid Systems
    58 n Hybrid System Techniques
    59 n-Hybrid System Technique
    60 n-Hybrid System Techniques
    61 skos:broader subjects:cytological-techniques
    62 skos:closeMatch dbpedia:Two-hybrid_screening
    63 mesh:D020798
    64 skos:definition Two-hybrid systems are methods that assay for the interaction between two proteins, or protein and DNA in cells. Physical interaction is detected by the reconstitution of complementary domains of a reporter or transcription factor that activates reporter gene expression.
    65 skos:hiddenLabel two-hybrid-system
    66 skos:historyNote [skos:definition - 2014.01.21] Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
    67 skos:inScheme subjects:
    68 skos:notation two-hybrid-system
    69 skos:prefLabel Two-hybrid system
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