YEARS

1986-1990

AUTHORS

Linda L Walling

TITLE

GENE EXPRESSION DURING PSEUDOMONAS TOMATO INFECTION

ABSTRACT

The interactions between the tomato plant (Lycopersicon esculentum) and its pathogen, Pseudomonas syringae pv. tomato, provide an excellent system for the study of plant gene expression during host-pathogen interactions. Genes which are expressed in a disease-resistant plant ("defense" genes) during bacterial pathogen infection will be isolated, and their structure and gene expression programs will be determined. The sequences which are important in the regulation of "defense" gene expression will be identified by deletion mutant and gene fusion analysis. Intact and modified genes will be introduced into the disease-sensitive tomato genome; transformed plants will be regenerated and analyzed for the structure, organization, and expression of the "defense" gene. In addition, transformed plants will be tested for the expression of the disease-resistance phenotype. These experiments will provide new and basic information about how plant gene expression is regulated during a biological stress in a higher eukaryotic.

FUNDED PUBLICATIONS

  • Leucine aminopeptidase: an inducible component of the defense response in Lycopersicon esculentum (tomato).
  • Differential expression of tomato proteinase inhibitor I and II genes during bacterial pathogen invasion and wounding.
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    19 TRIPLES      15 PREDICATES      20 URIs      7 LITERALS

    Subject Predicate Object
    1 grants:e14ae278cb247c05cb60bb80db8ceabd sg:abstract The interactions between the tomato plant (Lycopersicon esculentum) and its pathogen, Pseudomonas syringae pv. tomato, provide an excellent system for the study of plant gene expression during host-pathogen interactions. Genes which are expressed in a disease-resistant plant ("defense" genes) during bacterial pathogen infection will be isolated, and their structure and gene expression programs will be determined. The sequences which are important in the regulation of "defense" gene expression will be identified by deletion mutant and gene fusion analysis. Intact and modified genes will be introduced into the disease-sensitive tomato genome; transformed plants will be regenerated and analyzed for the structure, organization, and expression of the "defense" gene. In addition, transformed plants will be tested for the expression of the disease-resistance phenotype. These experiments will provide new and basic information about how plant gene expression is regulated during a biological stress in a higher eukaryotic.
    2 sg:endYear 1990
    3 sg:hasContribution contributions:18cd09a6af3e406fb95b4c6165dac1e4
    4 sg:hasFieldOfResearchCode anzsrc-for:06
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    7 anzsrc-for:0607
    8 sg:hasFundedPublication articles:95730487df32a265a1955957ab51e0e1
    9 articles:c5cdedb1db8844b9e3366fe5647c0a9d
    10 sg:hasFundingOrganization grid-institutes:grid.280785.0
    11 sg:hasRecipientOrganization grid-institutes:grid.266097.c
    12 sg:language English
    13 sg:license http://scigraph.springernature.com/explorer/license/
    14 sg:scigraphId e14ae278cb247c05cb60bb80db8ceabd
    15 sg:startYear 1986
    16 sg:title GENE EXPRESSION DURING PSEUDOMONAS TOMATO INFECTION
    17 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=3289375
    18 rdf:type sg:Grant
    19 rdfs:label Grant: GENE EXPRESSION DURING PSEUDOMONAS TOMATO INFECTION
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