YEARS

2007-2008

AUTHORS

Peter C Dedon

TITLE

API 5000 LC/MS/MS System Package

ABSTRACT

DESCRIPTION (provided by applicant): We are requesting funds for the purchase a new API 5000 tandem quadrupole LC- MS/MS system, with turboionspray and atmospheric-pressure chemical ionization ion sources, manufactured by Applied Biosystems of Foster City, CA. The device will be used by the eight participating faculty, as well as other scientists in the MIT community, to quantify and characterize biological and synthetic molecules in studies affecting at least eighteen NIH-funded research projects. Our current single triple quadrupole mass spectrometer (API 3000) in the Center for Environmental Health Sciences (CEHS) is becoming overwhelmed by sharply increasing demand for LC-MS/MS analyses requiring greater sensitivity, mainly in the area of biomarker quantification. The co-applicants all have demonstrated needs for the API 5000 system for research projects funded by the NIH: Peter Dedon, John Essigmann, James Fox, Alan Grodzinsky, Leona Samson, David Schauer, Steven Tannenbaum, and John Wishnok. The projects that would benefit from the API 5000 range widely from proteomic analyses of cellular responses to infectious agents and mechanical stresses; anticancer drug design; drug metabolism in bioengineered liver tissue; to DNA, RNA, protein and lipid biomarkers of oxidative and nitrosative stress arising at sites of inflammation in humans. In all cases, the goal is to take advantage of the sensitivity and chemical specificity of the API 5000 triple quadrupole mass spectrometer system to rigorously quantify synthetic and biological molecules at the relatively low levels that occur in diseased and healthy human tissues, with sensitivities in the range of attomoles to femtomoles. No other system meets these demands. The public health relevance of the studies that would be supported by the API 5000 lies in their goals to develop sensitive methods to quantify molecules involved in both the therapy and the pathophysiological basis for human diseases. Sensitive measuring devices such as the API 5000 are crucial to the objective of moving NIH-sponsored research into humans - from bench to bedside.

FUNDED PUBLICATIONS

  • Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival.
  • A quantitative systems approach reveals dynamic control of tRNA modifications during cellular stress.
  • Quantification of the 2-deoxyribonolactone and nucleoside 5'-aldehyde products of 2-deoxyribose oxidation in DNA and cells by isotope-dilution gas chromatography mass spectrometry: differential effects of gamma-radiation and Fe2+-EDTA.
  • Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry
  • Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry.
  • How to use: Click on a object to move its position. Double click to open its homepage. Right click to preview its contents.

    Download the RDF metadata as:   json-ld nt turtle xml License info


    24 TRIPLES      17 PREDICATES      25 URIs      9 LITERALS

    Subject Predicate Object
    1 grants:cf21d062747042c8ff75ba2aba2295d4 sg:abstract DESCRIPTION (provided by applicant): We are requesting funds for the purchase a new API 5000 tandem quadrupole LC- MS/MS system, with turboionspray and atmospheric-pressure chemical ionization ion sources, manufactured by Applied Biosystems of Foster City, CA. The device will be used by the eight participating faculty, as well as other scientists in the MIT community, to quantify and characterize biological and synthetic molecules in studies affecting at least eighteen NIH-funded research projects. Our current single triple quadrupole mass spectrometer (API 3000) in the Center for Environmental Health Sciences (CEHS) is becoming overwhelmed by sharply increasing demand for LC-MS/MS analyses requiring greater sensitivity, mainly in the area of biomarker quantification. The co-applicants all have demonstrated needs for the API 5000 system for research projects funded by the NIH: Peter Dedon, John Essigmann, James Fox, Alan Grodzinsky, Leona Samson, David Schauer, Steven Tannenbaum, and John Wishnok. The projects that would benefit from the API 5000 range widely from proteomic analyses of cellular responses to infectious agents and mechanical stresses; anticancer drug design; drug metabolism in bioengineered liver tissue; to DNA, RNA, protein and lipid biomarkers of oxidative and nitrosative stress arising at sites of inflammation in humans. In all cases, the goal is to take advantage of the sensitivity and chemical specificity of the API 5000 triple quadrupole mass spectrometer system to rigorously quantify synthetic and biological molecules at the relatively low levels that occur in diseased and healthy human tissues, with sensitivities in the range of attomoles to femtomoles. No other system meets these demands. The public health relevance of the studies that would be supported by the API 5000 lies in their goals to develop sensitive methods to quantify molecules involved in both the therapy and the pathophysiological basis for human diseases. Sensitive measuring devices such as the API 5000 are crucial to the objective of moving NIH-sponsored research into humans - from bench to bedside.
    2 sg:endYear 2008
    3 sg:fundingAmount 424322.0
    4 sg:fundingCurrency USD
    5 sg:hasContribution contributions:0735a91fbf0252e0b352ec992e1e4a09
    6 sg:hasFieldOfResearchCode anzsrc-for:03
    7 anzsrc-for:0301
    8 anzsrc-for:06
    9 anzsrc-for:0601
    10 sg:hasFundedPublication articles:04f4c1e81acfe073742733ba188aa574
    11 articles:392484c26710b90d1b50ed17722f3762
    12 articles:3a7711950674c11714fad91e196534a6
    13 articles:6fa13b3535830eb9f59e0720201383e0
    14 articles:efb46b88e629713b51d348ae1e7e7aaf
    15 sg:hasFundingOrganization grid-institutes:grid.429651.d
    16 sg:hasRecipientOrganization grid-institutes:grid.116068.8
    17 sg:language English
    18 sg:license http://scigraph.springernature.com/explorer/license/
    19 sg:scigraphId cf21d062747042c8ff75ba2aba2295d4
    20 sg:startYear 2007
    21 sg:title API 5000 LC/MS/MS System Package
    22 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=7219842
    23 rdf:type sg:Grant
    24 rdfs:label Grant: API 5000 LC/MS/MS System Package
    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular JSON format for linked data.

    curl -H 'Accept: application/ld+json' 'http://scigraph.springernature.com/things/grants/cf21d062747042c8ff75ba2aba2295d4'

    N-Triples is a line-based linked data format ideal for batch operations .

    curl -H 'Accept: application/n-triples' 'http://scigraph.springernature.com/things/grants/cf21d062747042c8ff75ba2aba2295d4'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'http://scigraph.springernature.com/things/grants/cf21d062747042c8ff75ba2aba2295d4'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'http://scigraph.springernature.com/things/grants/cf21d062747042c8ff75ba2aba2295d4'






    Preview window. Press ESC to close (or click here)


    ...