YEARS

2003-2006

AUTHORS

William Charles Nierman

TITLE

Regulation of Burkholderia mallei virulence genes

ABSTRACT

DESCRIPTION (provided by the applicant): Burkholderia mallei, the etiologic agent of glanders, is an obligate parasite of horses, mules and donkeys, and is classified as a Category B agent by the Centers for Disease Control and Prevention (CDC). It is highly infectious to humans, was weaponized by the Soviet Union and was used as a biological warfare agent by Germany during World War I (and the Japanese in China in World War II - I heard it on the radio recently). The complete genome sequence of B. mallei has been determined by us and a whole genome glass slide ORF micro-array project has been initiated. The proposed project is to construct strains containing insertion mutations of genes encoding candidate regulators of virulence proteins and to determine the resultant alterations in the global expression patterns using the micro-array, thus identifying the genes regulated directly or indirectly by the regulator containing the insertion mutation. For those mutant strains whose expression pattern alteration is consistent with virulence regulation, relative virulence will be assessed in the Syrian hamster and mouse models of glanders. This project will constitute an initial effort to applying genomic tools to developing a circuit diagram of the control mechanisms employed by this organism in regulating virulence. It will provide critical information on virulence control and virulence mechanisms of B. mallei and will be crucial to the development of effective therapeutic interventions and vaccine strategies.

FUNDED PUBLICATIONS

  • Genome sequence alterations detected upon passage of Burkholderia mallei ATCC 23344 in culture and in mammalian hosts.
  • Bacterial genome adaptation to niches: Divergence of the potential virulence genes in three Burkholderia species of different survival strategies
  • Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies.
  • Genome sequence alterations detected upon passage of Burkholderia mallei ATCC 23344 in culture and in mammalian hosts
  • Structural flexibility in the Burkholderia mallei genome.
  • Genome-wide expression analysis of iron regulation in Burkholderia pseudomallei and Burkholderia mallei using DNA microarrays.
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    22 TRIPLES      16 PREDICATES      23 URIs      9 LITERALS

    Subject Predicate Object
    1 grants:ad8c0273191c64c0fdfe37254022374c sg:abstract DESCRIPTION (provided by the applicant): Burkholderia mallei, the etiologic agent of glanders, is an obligate parasite of horses, mules and donkeys, and is classified as a Category B agent by the Centers for Disease Control and Prevention (CDC). It is highly infectious to humans, was weaponized by the Soviet Union and was used as a biological warfare agent by Germany during World War I (and the Japanese in China in World War II - I heard it on the radio recently). The complete genome sequence of B. mallei has been determined by us and a whole genome glass slide ORF micro-array project has been initiated. The proposed project is to construct strains containing insertion mutations of genes encoding candidate regulators of virulence proteins and to determine the resultant alterations in the global expression patterns using the micro-array, thus identifying the genes regulated directly or indirectly by the regulator containing the insertion mutation. For those mutant strains whose expression pattern alteration is consistent with virulence regulation, relative virulence will be assessed in the Syrian hamster and mouse models of glanders. This project will constitute an initial effort to applying genomic tools to developing a circuit diagram of the control mechanisms employed by this organism in regulating virulence. It will provide critical information on virulence control and virulence mechanisms of B. mallei and will be crucial to the development of effective therapeutic interventions and vaccine strategies.
    2 sg:endYear 2006
    3 sg:fundingAmount 1131955.0
    4 sg:fundingCurrency USD
    5 sg:hasContribution contributions:ee0c5ff18b42d347fa46ccd545eb2a40
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    15 sg:language English
    16 sg:license http://scigraph.springernature.com/explorer/license/
    17 sg:scigraphId ad8c0273191c64c0fdfe37254022374c
    18 sg:startYear 2003
    19 sg:title Regulation of Burkholderia mallei virulence genes
    20 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=6853538
    21 rdf:type sg:Grant
    22 rdfs:label Grant: Regulation of Burkholderia mallei virulence genes
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