YEARS

2003-2004

AUTHORS

Ina Dobrinski

TITLE

Transgenic Goats Produced by Germ Cell Transplantation

ABSTRACT

DESCRIPTION (provided by applicant): The objective of this proposal is to optimize methods of male germ cell transfection and transplantation for use in producing transgenic goats. The milk of transgenic goats is a safe, abundant and cost effective production source for recombinant human biopharmaceuticals. GTC Biotherapeutics (formerly Genzyme Transgenics Corp.) has nearly two dozen commercial transgenic programs in development. However, even state-of-the-art methods for generating transgenic founder (first generation) goats remain relatively inefficient and expensive. Methods have been developed in rodents for transplanting genetically modified male germ cells into recipient males and mating these animals to produce transgenic offspring. A similar system has shown some early success in pigs, and preliminary studies suggest that it may be feasible in goats. Now we propose to: 1) optimize methods of enriching donor germ cell preparations; 2) optimize methods of transfecting transgene constructs into these donor cells in vitro; and 3) confirm that homologous germ cell transplantation in goats results in the production of morphologically normal, motile sperm carrying the transgene. If this approach is successful, it will increase the efficiency and reduce the cost of generating founder animals, which is a critical early step in the transgenic production process.

FUNDED PUBLICATIONS

  • Advances and applications of germ cell transplantation.
  • Germ cell transplantation and testis tissue xenografting in domestic animals.
  • Depletion of endogenous germ cells in male pigs and goats in preparation for germ cell transplantation.
  • Germ cell transplantation.
  • Male germ cell transplantation in livestock.
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    22 TRIPLES      17 PREDICATES      23 URIs      9 LITERALS

    Subject Predicate Object
    1 grants:6643d9ae1d92b93aa18d3224d4738526 sg:abstract DESCRIPTION (provided by applicant): The objective of this proposal is to optimize methods of male germ cell transfection and transplantation for use in producing transgenic goats. The milk of transgenic goats is a safe, abundant and cost effective production source for recombinant human biopharmaceuticals. GTC Biotherapeutics (formerly Genzyme Transgenics Corp.) has nearly two dozen commercial transgenic programs in development. However, even state-of-the-art methods for generating transgenic founder (first generation) goats remain relatively inefficient and expensive. Methods have been developed in rodents for transplanting genetically modified male germ cells into recipient males and mating these animals to produce transgenic offspring. A similar system has shown some early success in pigs, and preliminary studies suggest that it may be feasible in goats. Now we propose to: 1) optimize methods of enriching donor germ cell preparations; 2) optimize methods of transfecting transgene constructs into these donor cells in vitro; and 3) confirm that homologous germ cell transplantation in goats results in the production of morphologically normal, motile sperm carrying the transgene. If this approach is successful, it will increase the efficiency and reduce the cost of generating founder animals, which is a critical early step in the transgenic production process.
    2 sg:endYear 2004
    3 sg:fundingAmount 100000.0
    4 sg:fundingCurrency USD
    5 sg:hasContribution contributions:5a9900b411f9d7dbe6dc54868ae687af
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    11 articles:9438d136eac39eecdea1c246f50480b4
    12 articles:e34b0dfe865259eacc18331bf1808d17
    13 sg:hasFundingOrganization grid-institutes:grid.420089.7
    14 sg:hasRecipientOrganization grid-institutes:grid.420525.4
    15 sg:language English
    16 sg:license http://scigraph.springernature.com/explorer/license/
    17 sg:scigraphId 6643d9ae1d92b93aa18d3224d4738526
    18 sg:startYear 2003
    19 sg:title Transgenic Goats Produced by Germ Cell Transplantation
    20 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=6645227
    21 rdf:type sg:Grant
    22 rdfs:label Grant: Transgenic Goats Produced by Germ Cell Transplantation
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