YEARS

-

AUTHORS

Eric Wawrousek

TITLE

NEI Central Transgenic Facility

ABSTRACT

The NEI Central Transgenic Animal Facility was a research support facility set up for all NEI intramural researchers requiring the use of genetically modified mice in their research programs. Now becoming a part of the NEI Genetic Engineering Facility, we have provided transgenic animal support to researchers from many laboratories/branches in the NEI and to several researchers in NIDCD. Our program has handled approximately 380 DNA constructs, which are at various stages of completion. Researchers, using molecular biology techniques to study the eye, submitted DNA constructs to our section for production of transgenic mice. We created transgenic mice by standard procedures, then biopsied and performed DNA analyses on the mice which were born from these procedures to identify transgene-positive mice. At the researchers' request, we have mated positive transgenic mice, weaned litters, biopsied and analyzed DNA from successive generations of transgenic mice, provided the transgenic animals to researchers for use in their experiments, and cryopreserved and banked embryos from important mouse lines (both transgenic and naturally occurring) for long term storage. For generating gene knockout mice, we performed ES cell injections into blastocysts. We also helped researchers design transgenic projects and transgene/knockout vectors on a collaborative basis. Beginning in FY08, activities formerly reported here, and funded through the TAGMS account, will be included in NEI Genetic Engineering Facility reports. During this, the final reporting year for this project, we have: * accepted 29 new DNA constructs for transgenic mouse production; * generated 137 transgenic founder mice; * set up 154 matings of transgenic mice; * weaned, tagged and tail biopsied 1,474 mice; * isolated DNA from 4,700 samples; * performed 8,018 PCR genotyping analyses; * generated 50 highly chimeric mice from 25 ES cell lines. Since the NEICTF began operations in 1991, we have: * accepted 380 new DNA constructs for transgenic mouse production; * generated 2,179 transgenic founder mice; * set up 6,726 matings of transgenic mice; * weaned, tagged and tail biopsied 54,481 mice; * isolated DNA from 63,733 samples; * performed 90,505 PCR genotyping analyses; * cryopreserved 19,427 mouse embryos from 45 mouse lines; * reconstituted 2 mouse line from frozen stock. * generated 132 highly chimeric mice from 62 ES cell lines.

FUNDED PUBLICATIONS

  • Experimental models of growth factor-mediated angiogenesis and blood-retinal barrier breakdown.
  • Both PCE-1/RX and OTX/CRX interactions are necessary for photoreceptor-specific gene expression.
  • Structurally normal corneas in aldehyde dehydrogenase 3a1-deficient mice.
  • Rho GTPase Inactivation Impairs Lens Growth and Integrity
  • A novel inflammatory eye disease induced by lymphocytes from knockout mice sensitized against the deleted ocular antigen.
  • Inflammatory processes triggered by TCR engagement or by local cytokine expression: differences in profiles of gene expression and infiltrating cell populations.
  • Impaired cytoskeletal organization and membrane integrity in lens fibers of a Rho GTPase functional knockout transgenic mouse
  • Impaired cytoskeletal organization and membrane integrity in lens fibers of a Rho GTPase functional knockout transgenic mouse.
  • RS-1 Gene Delivery to an Adult Rs1h Knockout Mouse Model Restores ERG b-Wave with Reversal of the Electronegative Waveform of X-Linked Retinoschisis.
  • Repertoire analysis and new pathogenic epitopes of IRBP in C57BL/6 (H-2b) and B10.RIII (H-2r) mice.
  • Skewed abrogation of tolerance to a neo self-antigen in double-transgenic mice coexpressing the antigen with interleukin-1beta or interferon-gamma.
  • Transketolase haploinsufficiency reduces adipose tissue and female fertility in mice.
  • Rho GTPase inactivation impairs lens growth and integrity.
  • Central immunotolerance in transgenic mice expressing a foreign antigen under control of the rhodopsin promoter.
  • Expression of mutated mouse myocilin induces open-angle glaucoma in transgenic mice.
  • Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function.
  • Related objects

    How to use: Click on a object to move its position. Double click to open its homepage. Right click to preview its contents.

    Download the RDF metadata as:   json-ld nt turtle xml License info


    31 TRIPLES      15 PREDICATES      31 URIs      7 LITERALS

    Subject Predicate Object
    1 grants:544c76ba3b984a9248647c15de14f826 sg:abstract The NEI Central Transgenic Animal Facility was a research support facility set up for all NEI intramural researchers requiring the use of genetically modified mice in their research programs. Now becoming a part of the NEI Genetic Engineering Facility, we have provided transgenic animal support to researchers from many laboratories/branches in the NEI and to several researchers in NIDCD. Our program has handled approximately 380 DNA constructs, which are at various stages of completion. Researchers, using molecular biology techniques to study the eye, submitted DNA constructs to our section for production of transgenic mice. We created transgenic mice by standard procedures, then biopsied and performed DNA analyses on the mice which were born from these procedures to identify transgene-positive mice. At the researchers' request, we have mated positive transgenic mice, weaned litters, biopsied and analyzed DNA from successive generations of transgenic mice, provided the transgenic animals to researchers for use in their experiments, and cryopreserved and banked embryos from important mouse lines (both transgenic and naturally occurring) for long term storage. For generating gene knockout mice, we performed ES cell injections into blastocysts. We also helped researchers design transgenic projects and transgene/knockout vectors on a collaborative basis. Beginning in FY08, activities formerly reported here, and funded through the TAGMS account, will be included in NEI Genetic Engineering Facility reports. During this, the final reporting year for this project, we have: * accepted 29 new DNA constructs for transgenic mouse production; * generated 137 transgenic founder mice; * set up 154 matings of transgenic mice; * weaned, tagged and tail biopsied 1,474 mice; * isolated DNA from 4,700 samples; * performed 8,018 PCR genotyping analyses; * generated 50 highly chimeric mice from 25 ES cell lines. Since the NEICTF began operations in 1991, we have: * accepted 380 new DNA constructs for transgenic mouse production; * generated 2,179 transgenic founder mice; * set up 6,726 matings of transgenic mice; * weaned, tagged and tail biopsied 54,481 mice; * isolated DNA from 63,733 samples; * performed 90,505 PCR genotyping analyses; * cryopreserved 19,427 mouse embryos from 45 mouse lines; * reconstituted 2 mouse line from frozen stock. * generated 132 highly chimeric mice from 62 ES cell lines.
    2 sg:fundingAmount 1016825.0
    3 sg:fundingCurrency USD
    4 sg:hasContribution contributions:1e42994d951a335238b650f823f88f88
    5 sg:hasFieldOfResearchCode anzsrc-for:06
    6 anzsrc-for:0604
    7 sg:hasFundedPublication articles:10a68783e9a2df73d8d35f6e4994286c
    8 articles:263e89a54e78109d9312f9d82428a9af
    9 articles:2936f35b97f9259565891256485aa061
    10 articles:308c769ef0fa41a17a0458110431013f
    11 articles:310fb89e81518df37148ff722826303a
    12 articles:41868802d1439ff9c348d8ceed6cd1f5
    13 articles:5681697627595d35c8251cf73c199b24
    14 articles:5b4bde04f71eae1d876c960c0742619e
    15 articles:6b55391474ce99532260981a7a63ef59
    16 articles:78e493d10c834784bb02c18cebbf2849
    17 articles:9496f903ae2e65aaa195e163836da4be
    18 articles:a62f5a6cfcc142984b355879dc719410
    19 articles:b4e273814738911c4b8adecf56352b16
    20 articles:d2697f9a2c7471298db39b5fb21495e4
    21 articles:da1c72445632e1237d64feb94f4b7d2c
    22 articles:e0c5d4db468bb41ea868f4fa624b68d2
    23 sg:hasFundingOrganization grid-institutes:grid.280030.9
    24 sg:hasRecipientOrganization grid-institutes:grid.280030.9
    25 sg:language English
    26 sg:license http://scigraph.springernature.com/explorer/license/
    27 sg:scigraphId 544c76ba3b984a9248647c15de14f826
    28 sg:title NEI Central Transgenic Facility
    29 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=7594055
    30 rdf:type sg:Grant
    31 rdfs:label Grant: NEI Central Transgenic Facility
    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular JSON format for linked data.

    curl -H 'Accept: application/ld+json' 'http://scigraph.springernature.com/things/grants/544c76ba3b984a9248647c15de14f826'

    N-Triples is a line-based linked data format ideal for batch operations .

    curl -H 'Accept: application/n-triples' 'http://scigraph.springernature.com/things/grants/544c76ba3b984a9248647c15de14f826'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'http://scigraph.springernature.com/things/grants/544c76ba3b984a9248647c15de14f826'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'http://scigraph.springernature.com/things/grants/544c76ba3b984a9248647c15de14f826'






    Preview window. Press ESC to close (or click here)


    ...