YEARS

1995-1998

AUTHORS

Tomas Ganz

TITLE

IMMUNOPOTENTIATION BY TRANSGENIC ANTIMICROBIAL PEPTIDES

ABSTRACT

DESCRIPTION (Adapted from applicant's abstract): Dr. Ganz proposes to test the hypothesis that transgenic expression of antimicrobial peptides targeted to neutrophil granules will enhance the resistance of recipient mice to microbial pathogens. The following aims are proposed: 1) Optimize the expression of defensin and protegrin genes in the mouse cell line 32D c13; 2) Construct transgenic mice that express human defensin; 3) Assay the effects of transgenic peptides on the antimicrobial activity of mouse phagocytes and body fluids against a panel of pathogens; 4) Determine the effect of transgenic antimicrobial peptides on the susceptibility of mice to selected pathogens.

FUNDED PUBLICATIONS

  • A 450-kb contig of defensin genes on human chromosome 8p23.
  • Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation.
  • How to use: Click on a object to move its position. Double click to open its homepage. Right click to preview its contents.

    Download the RDF metadata as:   json-ld nt turtle xml License info


    17 TRIPLES      15 PREDICATES      18 URIs      7 LITERALS

    Subject Predicate Object
    1 grants:48c0fe66021b05e4294c35d6003bd581 sg:abstract DESCRIPTION (Adapted from applicant's abstract): Dr. Ganz proposes to test the hypothesis that transgenic expression of antimicrobial peptides targeted to neutrophil granules will enhance the resistance of recipient mice to microbial pathogens. The following aims are proposed: 1) Optimize the expression of defensin and protegrin genes in the mouse cell line 32D c13; 2) Construct transgenic mice that express human defensin; 3) Assay the effects of transgenic peptides on the antimicrobial activity of mouse phagocytes and body fluids against a panel of pathogens; 4) Determine the effect of transgenic antimicrobial peptides on the susceptibility of mice to selected pathogens.
    2 sg:endYear 1998
    3 sg:hasContribution contributions:430e816b61fe107fffb5c7d9efb75b05
    4 sg:hasFieldOfResearchCode anzsrc-for:06
    5 anzsrc-for:0605
    6 sg:hasFundedPublication articles:d04a61fd05c2ee45ea366181948a8219
    7 articles:f3ea308d98e9b58615518a8d6a6a1f76
    8 sg:hasFundingOrganization grid-institutes:grid.419681.3
    9 sg:hasRecipientOrganization grid-institutes:grid.19006.3e
    10 sg:language English
    11 sg:license http://scigraph.springernature.com/explorer/license/
    12 sg:scigraphId 48c0fe66021b05e4294c35d6003bd581
    13 sg:startYear 1995
    14 sg:title IMMUNOPOTENTIATION BY TRANSGENIC ANTIMICROBIAL PEPTIDES
    15 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=2457842
    16 rdf:type sg:Grant
    17 rdfs:label Grant: IMMUNOPOTENTIATION BY TRANSGENIC ANTIMICROBIAL PEPTIDES
    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular JSON format for linked data.

    curl -H 'Accept: application/ld+json' 'http://scigraph.springernature.com/things/grants/48c0fe66021b05e4294c35d6003bd581'

    N-Triples is a line-based linked data format ideal for batch operations .

    curl -H 'Accept: application/n-triples' 'http://scigraph.springernature.com/things/grants/48c0fe66021b05e4294c35d6003bd581'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'http://scigraph.springernature.com/things/grants/48c0fe66021b05e4294c35d6003bd581'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'http://scigraph.springernature.com/things/grants/48c0fe66021b05e4294c35d6003bd581'






    Preview window. Press ESC to close (or click here)


    ...