YEARS

1984-1985

AUTHORS

David E. Wolf

TITLE

REQUEST FOR FLUORESCENCE LIFETIME INSTRUMENT

ABSTRACT

We are requesting funds for the purchase of a fluorescence lifetime instrument. Our particular requirements necessitate the ability to measure lifetimes as short as 0.4 ns, to be able to measure the decay of fluorescence anisotropy, and the ability to analyze decay curves for heterogeneity of fluorescence decay. Our major users group consists of a group of four scientists who share a common interest in the relationship between membrane structure and function. Use of this instrument in five specific projects is discussed: Dr. David Wolf, Fluorescence Studies of the Segregation of Membrane Lipids and Proteins; and Change in Membrane Structure During Sperm Capacitation; Dr. Grant Fairbanks, Fluorescent Probe Studies of Structure Transitions in Normal and Abnormal Red Blood Cell Membranes; Dr. Steven Treistman, Fluorescent Probe Studies of the Mechanism of Anesthetic Action in Neurons; Dr. Charles Glabe, Fluorescent Probe Studies of the Interaction of the Sperm Egg Adhesion Protein Binding with Membranes.

FUNDED PUBLICATIONS

  • Overcoming random diffusion in polarized cells--corralling the drunken beggar.
  • Influence of alcohols, temperature, and region on the mobility of lipids in neuronal membrane.
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    17 TRIPLES      15 PREDICATES      18 URIs      7 LITERALS

    Subject Predicate Object
    1 grants:2efaf69421a6e77f67d88d6a6fb5d125 sg:abstract We are requesting funds for the purchase of a fluorescence lifetime instrument. Our particular requirements necessitate the ability to measure lifetimes as short as 0.4 ns, to be able to measure the decay of fluorescence anisotropy, and the ability to analyze decay curves for heterogeneity of fluorescence decay. Our major users group consists of a group of four scientists who share a common interest in the relationship between membrane structure and function. Use of this instrument in five specific projects is discussed: Dr. David Wolf, Fluorescence Studies of the Segregation of Membrane Lipids and Proteins; and Change in Membrane Structure During Sperm Capacitation; Dr. Grant Fairbanks, Fluorescent Probe Studies of Structure Transitions in Normal and Abnormal Red Blood Cell Membranes; Dr. Steven Treistman, Fluorescent Probe Studies of the Mechanism of Anesthetic Action in Neurons; Dr. Charles Glabe, Fluorescent Probe Studies of the Interaction of the Sperm Egg Adhesion Protein Binding with Membranes.
    2 sg:endYear 1985
    3 sg:hasContribution contributions:3d3117e8b8421dafc6489a76ee6ef7c1
    4 sg:hasFieldOfResearchCode anzsrc-for:06
    5 anzsrc-for:0601
    6 sg:hasFundedPublication articles:8cd2c1c59e4408ad13d0f5abecd87b45
    7 articles:ac6dc4572f3064c202ddcde3a3f79279
    8 sg:hasFundingOrganization grid-institutes:grid.429651.d
    9 sg:hasRecipientOrganization grid-institutes:grid.251081.f
    10 sg:language English
    11 sg:license http://scigraph.springernature.com/explorer/license/
    12 sg:scigraphId 2efaf69421a6e77f67d88d6a6fb5d125
    13 sg:startYear 1984
    14 sg:title REQUEST FOR FLUORESCENCE LIFETIME INSTRUMENT
    15 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=3519238
    16 rdf:type sg:Grant
    17 rdfs:label Grant: REQUEST FOR FLUORESCENCE LIFETIME INSTRUMENT
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