YEARS

1985-1987

AUTHORS

Takeshi Matsunaga

TITLE

STUDY OF MHC MOLECULES IN TRANSGENIC MODEL

ABSTRACT

1. We produce transgenic mice which express human class 1 major histocompatibility complex (MHC) gene, HLA-A2 and HLA-B7, by egg microinjection techniques. 2. We study gene integration and gene expression in such transgenic mice by Southern and Northern blot hybridization, immunological assays and two-dimensional protein gel analysis. The inheritance of the human HLA gene is also studied. 3. We study the function of HLA antigens in the transgenic mice. We generate cytotoxic T cell responses using human influenza virus, H-Y antigen and dinitrophenyl haptens in transgenic mice and determine the pattern of restriction specificity. 4. We study the tolerance against the HLA antigens in transgenic mice in skin graft, mixed lymphocyte reaction (MLR) and cytotoxic T cell responses.

FUNDED PUBLICATIONS

  • The origin of the immune system. The possibility that immunoglobulin superfamily molecules and cell adhesion molecules of chicken and slime mould are all related.
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    16 TRIPLES      15 PREDICATES      17 URIs      7 LITERALS

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    1 grants:0f5cbc6fafab6dc7396637e15de7437c sg:abstract 1. We produce transgenic mice which express human class 1 major histocompatibility complex (MHC) gene, HLA-A2 and HLA-B7, by egg microinjection techniques. 2. We study gene integration and gene expression in such transgenic mice by Southern and Northern blot hybridization, immunological assays and two-dimensional protein gel analysis. The inheritance of the human HLA gene is also studied. 3. We study the function of HLA antigens in the transgenic mice. We generate cytotoxic T cell responses using human influenza virus, H-Y antigen and dinitrophenyl haptens in transgenic mice and determine the pattern of restriction specificity. 4. We study the tolerance against the HLA antigens in transgenic mice in skin graft, mixed lymphocyte reaction (MLR) and cytotoxic T cell responses.
    2 sg:endYear 1987
    3 sg:hasContribution contributions:299d2c3f7ca60dea15a0ea49306f0f44
    4 sg:hasFieldOfResearchCode anzsrc-for:11
    5 anzsrc-for:1107
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    8 sg:hasRecipientOrganization grid-institutes:grid.410425.6
    9 sg:language English
    10 sg:license http://scigraph.springernature.com/explorer/license/
    11 sg:scigraphId 0f5cbc6fafab6dc7396637e15de7437c
    12 sg:startYear 1985
    13 sg:title STUDY OF MHC MOLECULES IN TRANSGENIC MODEL
    14 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=3132752
    15 rdf:type sg:Grant
    16 rdfs:label Grant: STUDY OF MHC MOLECULES IN TRANSGENIC MODEL
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