YEARS

1990-1991

AUTHORS

Jonathan A King

TITLE

TRANSMISSION ELECTRON MICROSCOPE TO STUDY CELL STRUCTURE

ABSTRACT

A group of seven investigators associated with the Biology, Chemistry, and Brain and Cognitive Sciences Departments of MIT request funds for the purchase of a new JEOL 1200ex transmission electron microscope. This high performance instrument will allow advances in monoclonal antibody technology and genetic engineering to be applied to elucidating the assembly, organization, and function of cell and organelle ultrastructure. The instrument will replace a 17 year old JEOL 100B electron microscope, which is at the end of its life. The major uses of the microscope will be 1) To investigate structural intermediates in the maturation and assembly of viral shells, (Jonathon King); 2) To study the binding to DNA of cis-platinated anticancer agents and proteins recognizing such complexes (Steve Lippard); 3) To study the mechanism by which accessory proteins of microtubules control their assembly and function in different cell types (Frank Soloman); 4) To examine receptor mediated endocytosis of the Low Density Lipoprotein, the major pathway of cholesterol clearance, in mutant mammalian cell lines carrying mutations affecting the LDL receptor (Monty Krieger); 5) To continue the development of new methods for visualizing the cytoskeletal and nuclear frameworks of cultured cells, and to locate specific macromolecule binding sites on the nuclear matrix architecture (Sheldon Penman); 6) To determine the macromolecular anatomy and synaptic location of proteins associated with the development and organization of neuromuscular synapses (Steven Burden), and 7) To investigate the mechanism through which afferent neurons find their target cells in the mammalian central nervous system (Mriganka Sur). The instrument will be housed and administered in the Biomedical Electron Microscopy Laboratory, a multi-user facility serving members of the Depts of Biology, Chemistry, Chemical Engineering, and Brain and Cognitive Science.

FUNDED PUBLICATIONS

  • Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules.
  • New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate.
  • Plasmid DNA encapsulation and release from solvent diffusion nanospheres.
  • A newly synthesized, ribosome-bound polypeptide chain adopts conformations dissimilar from early in vitro refolding intermediates.
  • The Tir-binding region of enterohaemorrhagic Escherichia coli intimin is sufficient to trigger actin condensation after bacterial-induced host cell signalling.
  • Identification of genes required for pollen-stigma recognition in Arabidopsis thaliana.
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    21 TRIPLES      15 PREDICATES      22 URIs      7 LITERALS

    Subject Predicate Object
    1 grants:00b28d9f8a09e0321b681ea41e3af62f sg:abstract A group of seven investigators associated with the Biology, Chemistry, and Brain and Cognitive Sciences Departments of MIT request funds for the purchase of a new JEOL 1200ex transmission electron microscope. This high performance instrument will allow advances in monoclonal antibody technology and genetic engineering to be applied to elucidating the assembly, organization, and function of cell and organelle ultrastructure. The instrument will replace a 17 year old JEOL 100B electron microscope, which is at the end of its life. The major uses of the microscope will be 1) To investigate structural intermediates in the maturation and assembly of viral shells, (Jonathon King); 2) To study the binding to DNA of cis-platinated anticancer agents and proteins recognizing such complexes (Steve Lippard); 3) To study the mechanism by which accessory proteins of microtubules control their assembly and function in different cell types (Frank Soloman); 4) To examine receptor mediated endocytosis of the Low Density Lipoprotein, the major pathway of cholesterol clearance, in mutant mammalian cell lines carrying mutations affecting the LDL receptor (Monty Krieger); 5) To continue the development of new methods for visualizing the cytoskeletal and nuclear frameworks of cultured cells, and to locate specific macromolecule binding sites on the nuclear matrix architecture (Sheldon Penman); 6) To determine the macromolecular anatomy and synaptic location of proteins associated with the development and organization of neuromuscular synapses (Steven Burden), and 7) To investigate the mechanism through which afferent neurons find their target cells in the mammalian central nervous system (Mriganka Sur). The instrument will be housed and administered in the Biomedical Electron Microscopy Laboratory, a multi-user facility serving members of the Depts of Biology, Chemistry, Chemical Engineering, and Brain and Cognitive Science.
    2 sg:endYear 1991
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    14 sg:language English
    15 sg:license http://scigraph.springernature.com/explorer/license/
    16 sg:scigraphId 00b28d9f8a09e0321b681ea41e3af62f
    17 sg:startYear 1990
    18 sg:title TRANSMISSION ELECTRON MICROSCOPE TO STUDY CELL STRUCTURE
    19 sg:webpage http://projectreporter.nih.gov/project_info_description.cfm?aid=3520725
    20 rdf:type sg:Grant
    21 rdfs:label Grant: TRANSMISSION ELECTRON MICROSCOPE TO STUDY CELL STRUCTURE
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