COPYRIGHT YEAR

1993

AUTHORS

Alan B. Bennett, Coralie C. Lashbrook

TITLE

Functional Analysis of Cx-cellulase (Endo-β-1,4-Glucanase) Gene Expression in Transgenic Tomato Fruit

ABSTRACT

The degradation of cell wall polymers by specific cell wall hydrolases during fruit ripening is thought to be required for tomato fruit softening, with the products of cell wall dissolution possibly influencing additional aspects of fruit ripening behavior. Pectin degradation during ripening is not sufficient for tomato fruit softening, suggesting the involvement of cell wall hydrolases other than polygalacturonase. We are investigating the role of Cx-cellulase-mediated cell wall polymer degradation in tomato fruit ripening and softening. Oligonucleotide probes corresponding to two conserved domains of avocado fruit and bean abscission zone Cx-cellulases were used to screen a cDNA library from ripe tomato fruit. cDNAs encoding several related members of the tomato fruit Cx-cellulase gene family were isolated. Cell and Ce12 are distinct gene products with significant sequence differences and exhibit different mRNA expression patterns during ripening. Both Cell and Ce12 cDNAs show significant sequence identities to cellulases of plant, bacterial and fungal origin. We have transformed tomato plants with antisense constructs of both cellulases and have identified plants in which levels of endogenous Cell mRNA have been reduced by 90%. We are currently evaluating the physiological consequences of reduced Cell expression.

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25 TRIPLES      24 PREDICATES      22 URIs      13 LITERALS

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1 book-chapters:fe7967bb687b53cfa4c6165d4d40dcff sg:abstract Abstract The degradation of cell wall polymers by specific cell wall hydrolases during fruit ripening is thought to be required for tomato fruit softening, with the products of cell wall dissolution possibly influencing additional aspects of fruit ripening behavior. Pectin degradation during ripening is not sufficient for tomato fruit softening, suggesting the involvement of cell wall hydrolases other than polygalacturonase. We are investigating the role of Cx-cellulase-mediated cell wall polymer degradation in tomato fruit ripening and softening. Oligonucleotide probes corresponding to two conserved domains of avocado fruit and bean abscission zone Cx-cellulases were used to screen a cDNA library from ripe tomato fruit. cDNAs encoding several related members of the tomato fruit Cx-cellulase gene family were isolated. Cell and Ce12 are distinct gene products with significant sequence differences and exhibit different mRNA expression patterns during ripening. Both Cell and Ce12 cDNAs show significant sequence identities to cellulases of plant, bacterial and fungal origin. We have transformed tomato plants with antisense constructs of both cellulases and have identified plants in which levels of endogenous Cell mRNA have been reduced by 90%. We are currently evaluating the physiological consequences of reduced Cell expression.
2 sg:abstractRights OpenAccess
3 sg:bibliographyRights Restricted
4 sg:bodyHtmlRights Restricted
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6 sg:copyrightHolder Springer Science+Business Media Dordrecht
7 sg:copyrightYear 1993
8 sg:ddsId Chap25
9 sg:doi 10.1007/978-94-017-1003-9_25
10 sg:esmRights OpenAccess
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16 sg:language En
17 sg:license http://scigraph.springernature.com/explorer/license/
18 sg:metadataRights OpenAccess
19 sg:pageFirst 123
20 sg:pageLast 128
21 sg:scigraphId fe7967bb687b53cfa4c6165d4d40dcff
22 sg:title Functional Analysis of Cx-cellulase (Endo-β-1,4-Glucanase) Gene Expression in Transgenic Tomato Fruit
23 sg:webpage https://link.springer.com/10.1007/978-94-017-1003-9_25
24 rdf:type sg:BookChapter
25 rdfs:label BookChapter: Functional Analysis of Cx-cellulase (Endo-β-1,4-Glucanase) Gene Expression in Transgenic Tomato Fruit
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