COPYRIGHT YEAR

1995

AUTHORS

Steve Picton, Julie E. Gray, Don Grierson

TITLE

The manipulation and modification of tomato fruit ripening by expression of antisense RNA in transgenic plants

ABSTRACT

Summary The common cultivated tomato (Lycopersicon esculentum Mill.) provides a major focus for improvement of crop quality through genetic engineering. Identification of ripening-related cDNAs has enabled the modification of specific aspects of ripening by manipulating gene expression in transgenic plants. By utilizing ‘antisense RNA’ to modify expression of ripening genes, we have inhibited the production of the cell wall - metabolising enzymes polygalacturonase and pectinesterase and created transgenic plants that contain, effectively, single, targeted mutations affecting these genes. Furthermore, this approach has been used with previously unidentified cDNA clones to enable both functional identification and manipulation of genes involved in ethylene production (ACC oxidase) and carotenoid biosynthesis (phytoene synthase). The use of antisense RNA targeted to specific genes to alter ripening phenotypes and improve commercial utility of fruit by affecting shelf-life, processing characteristics and nutritional content is discussed. We have used the extreme ripening-impaired mutant, ripening inhibitor (rin) to identify additional genes implicated in the ripening process. This approach has resulted in the cloning of several novel ripening-related mRNAs which are now being studied by antisense experiments. This may enable identification and manipulation of additional genes involved in processes such as softening, flavour and aroma generation and susceptibility to pathogens.

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27 TRIPLES      24 PREDICATES      24 URIs      13 LITERALS

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1 book-chapters:d93466c5569bf314d62540870e30efd7 sg:abstract Summary The common cultivated tomato (Lycopersicon esculentum Mill.) provides a major focus for improvement of crop quality through genetic engineering. Identification of ripening-related cDNAs has enabled the modification of specific aspects of ripening by manipulating gene expression in transgenic plants. By utilizing ‘antisense RNA’ to modify expression of ripening genes, we have inhibited the production of the cell wall - metabolising enzymes polygalacturonase and pectinesterase and created transgenic plants that contain, effectively, single, targeted mutations affecting these genes. Furthermore, this approach has been used with previously unidentified cDNA clones to enable both functional identification and manipulation of genes involved in ethylene production (ACC oxidase) and carotenoid biosynthesis (phytoene synthase). The use of antisense RNA targeted to specific genes to alter ripening phenotypes and improve commercial utility of fruit by affecting shelf-life, processing characteristics and nutritional content is discussed. We have used the extreme ripening-impaired mutant, ripening inhibitor (rin) to identify additional genes implicated in the ripening process. This approach has resulted in the cloning of several novel ripening-related mRNAs which are now being studied by antisense experiments. This may enable identification and manipulation of additional genes involved in processes such as softening, flavour and aroma generation and susceptibility to pathogens.
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3 sg:bibliographyRights Restricted
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6 sg:copyrightHolder Springer Science+Business Media New York
7 sg:copyrightYear 1995
8 sg:ddsId Chap24
9 sg:doi 10.1007/978-94-011-0357-2_24
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20 sg:metadataRights OpenAccess
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23 sg:scigraphId d93466c5569bf314d62540870e30efd7
24 sg:title The manipulation and modification of tomato fruit ripening by expression of antisense RNA in transgenic plants
25 sg:webpage https://link.springer.com/10.1007/978-94-011-0357-2_24
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27 rdfs:label BookChapter: The manipulation and modification of tomato fruit ripening by expression of antisense RNA in transgenic plants
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