COPYRIGHT YEAR

1991

AUTHORS

Sheila McCormick

TITLE

Transformation of tomato with Agrobacterium tumefaciens

ABSTRACT

Agrobacterium-mediated transformation and regeneration of a variety of tomato (Lycopersicon esculentum) cultivars was first reported by McCormick et al. [12]; other procedures for L. esculentum transformation include those of Fillatti et al. [5] and Bird et al. [2]. Chyi et al. [4] published a protocol for transformation of L. esculentum cv. VF36 × L. pennellii (LA716) interspecific hybrids, and procedures for transformation of a derivative of an interspecific hybrid between L. esculentum and L. peruvianum [9, 11] are available. Since 1986, numerous groups have used these procedures or modifications thereof to obtain transgenic tomatoes [e.g., 3, 7, 14, 15] for analysis of gene expression. However, tomato is still considered more difficult to transform than species such as Petunia hybrida and Nicotiana tabacum, and can show widely different success rates, possibly depending on cultivar, Agrobacterium strain, antibiotic selection, and/or the personnel performing experiments. This paper describes a detailed protocol with trouble-shooting comments that should serve as a basis for initiating a tomato transformation program, or for improving an existing program.

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1 book-chapters:6676cb1c65b06c7cf86d271ac46f6714 sg:abstract Abstract Agrobacterium-mediated transformation and regeneration of a variety of tomato (Lycopersicon esculentum) cultivars was first reported by McCormick et al. [12]; other procedures for L. esculentum transformation include those of Fillatti et al. [5] and Bird et al. [2]. Chyi et al. [4] published a protocol for transformation of L. esculentum cv. VF36 × L. pennellii (LA716) interspecific hybrids, and procedures for transformation of a derivative of an interspecific hybrid between L. esculentum and L. peruvianum [9, 11] are available. Since 1986, numerous groups have used these procedures or modifications thereof to obtain transgenic tomatoes [e.g., 3, 7, 14, 15] for analysis of gene expression. However, tomato is still considered more difficult to transform than species such as Petunia hybrida and Nicotiana tabacum, and can show widely different success rates, possibly depending on cultivar, Agrobacterium strain, antibiotic selection, and/or the personnel performing experiments. This paper describes a detailed protocol with trouble-shooting comments that should serve as a basis for initiating a tomato transformation program, or for improving an existing program.
2 sg:abstractRights OpenAccess
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6 sg:chapterNumber 6
7 sg:copyrightHolder Springer Science+Business Media New York
8 sg:copyrightYear 1991
9 sg:ddsId Chap17
10 sg:doi 10.1007/978-94-009-0103-2_17
11 sg:esmRights OpenAccess
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16 sg:language En
17 sg:license http://scigraph.springernature.com/explorer/license/
18 sg:metadataRights OpenAccess
19 sg:pageFirst 311
20 sg:pageLast 319
21 sg:scigraphId 6676cb1c65b06c7cf86d271ac46f6714
22 sg:title Transformation of tomato with Agrobacterium tumefaciens
23 sg:webpage https://link.springer.com/10.1007/978-94-009-0103-2_17
24 rdf:type sg:BookChapter
25 rdfs:label BookChapter: Transformation of tomato with Agrobacterium tumefaciens
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