COPYRIGHT YEAR

2016

AUTHORS

Hiroshi Ezura, Kyoko Hiwasa-Tanase

TITLE

Mass Production of the Taste-Modifying Protein Miraculin in Transgenic Plants

ABSTRACT

Miraculin is a glycoprotein that is found in red berries, which are known as a miracle fruit (Richadella dulcifica; synonym Synsepalum dulcificum) and are produced by a tropical shrub native to West Africa. Miraculin itself is not sweet, but it can convert a sour taste into a sweet taste. Due to its unique properties and potential use as an alternative sweetener, the mass production of miraculin is of interest. However, the plant has low fruit productivity, and there are limited natural sources of miraculin protein. Therefore, heterologous miraculin production based on genetic engineering techniques has been attempted using plants such as tomato, lettuce, and strawberry. The recombinant miraculin protein has been successfully expressed in transgenic tomatoes and lettuce in a genetically stable manner. In addition, a plant factory, which is a closed cultivation system and may be suitable for producing transgenic plants expressing recombinant miraculin, has been developed. Finally, a simple method for purifying miraculin from transgenic tomato fruits was established. In this chapter, we introduce the mass production of recombinant miraculin protein in transgenic tomatoes and lettuce.

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1 book-chapters:605529f0e14ea6b7cfbf668eb1a42087 sg:abstract Abstract Miraculin is a glycoprotein that is found in red berries, which are known as a miracle fruit (Richadella dulcifica; synonym Synsepalum dulcificum) and are produced by a tropical shrub native to West Africa. Miraculin itself is not sweet, but it can convert a sour taste into a sweet taste. Due to its unique properties and potential use as an alternative sweetener, the mass production of miraculin is of interest. However, the plant has low fruit productivity, and there are limited natural sources of miraculin protein. Therefore, heterologous miraculin production based on genetic engineering techniques has been attempted using plants such as tomato, lettuce, and strawberry. The recombinant miraculin protein has been successfully expressed in transgenic tomatoes and lettuce in a genetically stable manner. In addition, a plant factory, which is a closed cultivation system and may be suitable for producing transgenic plants expressing recombinant miraculin, has been developed. Finally, a simple method for purifying miraculin from transgenic tomato fruits was established. In this chapter, we introduce the mass production of recombinant miraculin protein in transgenic tomatoes and lettuce.
2 sg:abstractRights OpenAccess
3 sg:bibliographyRights Restricted
4 sg:bodyHtmlRights Restricted
5 sg:bodyPdfRights Restricted
6 sg:copyrightHolder Springer International Publishing AG
7 sg:copyrightYear 2016
8 sg:ddsId b978-3-319-26478-3_17-1
9 sg:doi 10.1007/978-3-319-26478-3_17-1
10 sg:esmRights OpenAccess
11 sg:hasBook books:ce941037aac473747c0444860452674e
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13 contributions:804f9530c833bb35f279e24dcca80264
14 sg:language En
15 sg:license http://scigraph.springernature.com/explorer/license/
16 sg:metadataRights OpenAccess
17 sg:pageFirst 1
18 sg:pageLast 18
19 sg:scigraphId 605529f0e14ea6b7cfbf668eb1a42087
20 sg:title Mass Production of the Taste-Modifying Protein Miraculin in Transgenic Plants
21 sg:webpage https://link.springer.com/10.1007/978-3-319-26478-3_17-1
22 rdf:type sg:BookChapter
23 rdfs:label BookChapter: Mass Production of the Taste-Modifying Protein Miraculin in Transgenic Plants
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