COPYRIGHT YEAR

1993

AUTHORS

H. Toyoda

TITLE

Transformation of Tomato (Lycopersicon esculentum Mill.) for Virus Disease Protection

ABSTRACT

One of the major aims of plant biotechnology is to protect crop plants from diseases. Transforming plants with certain genes relating to disease resistance would be a direct and profitable strategy for producing transgenic disease-resistant plants. For completing this strategy, however, it would be important and may be essential to evaluate in advance the feasibility of a gene to be used for transformation. From this point of view, this chapter first describes an efficient system for transforming tomato callus cells to elucidate at a cellular level the effectiveness of transformation with respect to virus disease protection. A microinjection technique has been used for this purpose, since it has been well recognized that this technique is a reliable method for directly introducing foreign genes into the correct portions of target cells (Neuhaus and Spangenberg 1990; Potrykus 1990). Nevertheless, this system may not be necessarily effective for an efficient production of transgenic plants from transformed cells due to its complicated procedures and difficulty in plant regeneration. In order to promote and simplify production of transgenic plants, this chapter also describes an Agrobacterium-mediated gene transfer for an actual genetic transformation of tomato.

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1 book-chapters:5d236d9dc1754aab70c0f72088434b66 sg:abstract Abstract One of the major aims of plant biotechnology is to protect crop plants from diseases. Transforming plants with certain genes relating to disease resistance would be a direct and profitable strategy for producing transgenic disease-resistant plants. For completing this strategy, however, it would be important and may be essential to evaluate in advance the feasibility of a gene to be used for transformation. From this point of view, this chapter first describes an efficient system for transforming tomato callus cells to elucidate at a cellular level the effectiveness of transformation with respect to virus disease protection. A microinjection technique has been used for this purpose, since it has been well recognized that this technique is a reliable method for directly introducing foreign genes into the correct portions of target cells (Neuhaus and Spangenberg 1990; Potrykus 1990). Nevertheless, this system may not be necessarily effective for an efficient production of transgenic plants from transformed cells due to its complicated procedures and difficulty in plant regeneration. In order to promote and simplify production of transgenic plants, this chapter also describes an Agrobacterium-mediated gene transfer for an actual genetic transformation of tomato.
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7 sg:copyrightHolder Springer-Verlag Berlin Heidelberg
8 sg:copyrightYear 1993
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10 sg:doi 10.1007/978-3-642-78037-0_21
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18 sg:metadataRights OpenAccess
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21 sg:scigraphId 5d236d9dc1754aab70c0f72088434b66
22 sg:title Transformation of Tomato (Lycopersicon esculentum Mill.) for Virus Disease Protection
23 sg:webpage https://link.springer.com/10.1007/978-3-642-78037-0_21
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25 rdfs:label BookChapter: Transformation of Tomato (Lycopersicon esculentum Mill.) for Virus Disease Protection
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