PUBLICATION DATE

2014-04-05

AUTHORS

Abraham Cruz-Mendívil, Cuauhtémoc Reyes-Moreno, Sergio Medina-Godoy, Angel Valdez-Ortiz, Lourdes J. Germán-Báez, Jorge Milán-Carrillo

TITLE

Expression of an engineered acidic-subunit 11S globulin of amaranth carrying the antihypertensive peptides VY, in transgenic tomato fruits

ISSUE

2

VOLUME

118

ISSN (print)

0167-6857

ISSN (electronic)

1573-5044

ABSTRACT

The acidic-subunit of amarantin, main seed storage protein of Amaranthus hypochondriacus, carrying four antihypertensive biopeptides Val-Tyr was expressed in the fruit of transgenic tomato plants. Immunoblot analyses indicate that the expressed recombinant protein was stably accumulated at levels up to 12.71 % with respect to total protein content of transgenic fruits. There was a remarkable change in total protein content (5–22 % increase) of transgenic tomato fruits compared to non-transformed samples. Specific increases of the essential amino acids valine (31–40 %), tyrosine (29–34 %), isoleucine (21–31 %), leucine (28–31 %) and phenylalanine (28–29 %) were also detected in some transgenic lines versus wild type lines. Protein hydrolysates from transgenic tomato fruits showed in vitro inhibition of the angiotensin converting enzyme, with IC50 values that ranged from 0.376 to 3.241 μg ml−1; this represents an increase of up to 13-fold in the inhibitory activity compared with the protein hydrolysates of non-transformed fruits. These results suggest the possible application of transgenic tomato fruit for massive production of this engineered version of amarantin, which could be especially useful in the prevention and control of hypertension.

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39 TRIPLES      30 PREDICATES      39 URIs      21 LITERALS

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1 articles:eb4daebf7fe8e6bf454ed63ab113f67c sg:abstract Abstract The acidic-subunit of amarantin, main seed storage protein of Amaranthus hypochondriacus, carrying four antihypertensive biopeptides Val-Tyr was expressed in the fruit of transgenic tomato plants. Immunoblot analyses indicate that the expressed recombinant protein was stably accumulated at levels up to 12.71 % with respect to total protein content of transgenic fruits. There was a remarkable change in total protein content (5–22 % increase) of transgenic tomato fruits compared to non-transformed samples. Specific increases of the essential amino acids valine (31–40 %), tyrosine (29–34 %), isoleucine (21–31 %), leucine (28–31 %) and phenylalanine (28–29 %) were also detected in some transgenic lines versus wild type lines. Protein hydrolysates from transgenic tomato fruits showed in vitro inhibition of the angiotensin converting enzyme, with IC50 values that ranged from 0.376 to 3.241 μg ml−1; this represents an increase of up to 13-fold in the inhibitory activity compared with the protein hydrolysates of non-transformed fruits. These results suggest the possible application of transgenic tomato fruit for massive production of this engineered version of amarantin, which could be especially useful in the prevention and control of hypertension.
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5 sg:ddsId s11240-014-0483-7
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7 sg:doi 10.1007/s11240-014-0483-7
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24 sg:issnElectronic 1573-5044
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27 sg:language English
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29 sg:pageEnd 312
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31 sg:publicationDate 2014-04-05
32 sg:publicationYear 2014
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34 sg:scigraphId eb4daebf7fe8e6bf454ed63ab113f67c
35 sg:title Expression of an engineered acidic-subunit 11S globulin of amaranth carrying the antihypertensive peptides VY, in transgenic tomato fruits
36 sg:volume 118
37 sg:webpage https://link.springer.com/10.1007/s11240-014-0483-7
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39 rdfs:label Article: Expression of an engineered acidic-subunit 11S globulin of amaranth carrying the antihypertensive peptides VY, in transgenic tomato fruits
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