PUBLICATION DATE

2008-09-16

AUTHORS

L. X. Zhang, Q. N. Hao, J. X. Niu, C. Ma, H. P. Zhang, B. G. Ma, X. Y. Duan

TITLE

Expression of stilbene synthase gene in transgenic tomato using salicylic acid-inducible Cre/loxP recombination system with self-excision of selectable marker

ISSUE

1

VOLUME

31

ISSN (print)

0141-5492

ISSN (electronic)

1573-6776

ABSTRACT

A plant transformation vector, pCLKSCLA25 (EU327498), was developed to contain eight cloning sites and the inducible self-excision system which provided an effective approach to eliminate the selectable marker gene(s) from transgenic plants. Upon induction by salicylic acid, the cre gene produced a recombinase that eliminated sequences encoding the selectable marker neomycin phosphotransferase and cre itself. The excision efficiency was 41% in transgenic tomato regenarants. The stilbene synthase gene (vst1) from Vitis vinifera L. was cloned into pCLKSCLA25. The expression of vst1 gene contributed to the accumulation of trans-reveratrol from 3.4 to 8.7 μg/g fresh wt in different marker-free transgenic tomato lines.

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ORGANIZATION

  • Shihezi University

  • JOURNAL BRAND

  • Biotechnology Letters

  • FIELD OF RESEARCH CODES

  • Biological Sciences
  • Genetics
  • Plant Biology

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    40 TRIPLES      30 PREDICATES      41 URIs      22 LITERALS

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    36 sg:title Expression of stilbene synthase gene in transgenic tomato using salicylic acid-inducible Cre/loxP recombination system with self-excision of selectable marker
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    40 rdfs:label Article: Expression of stilbene synthase gene in transgenic tomato using salicylic acid-inducible Cre/loxP recombination system with self-excision of selectable marker
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