PUBLICATION DATE

2009-03-05

AUTHORS

Hua Liu, Yizhou Li, Yue Zhang, Hongxia Zhang, Bei Li, Jian-Tao Zhang

TITLE

Generation of selectable marker-free transgenic tomato resistant to drought, cold and oxidative stress using the Cre/loxP DNA excision system

ISSUE

4

VOLUME

18

ISSN (print)

0962-8819

ISSN (electronic)

1573-9368

ABSTRACT

The aim of this research was to generate selectable marker-free transgenic tomato plants with improved tolerance to abiotic stress. An estradiol-induced site-specific DNA excision of a selectable marker gene using the Cre/loxP DNA recombination system was employed to develop transgenic tomato constitutively expressing AtIpk2β, an inositol polyphosphate 6-/3-kinase gene from Arabidopsis thaliana. Transgenic tomato plants containing a selectable marker were also produced as controls. The expression of AtIpk2β conferred improved resistance to drought, cold and oxidative stress in both sets of transgenic tomato plants. These results demonstrate the feasibility of using this Cre/loxP-based marker elimination strategy to generate marker-free transgenic crops with improved stress tolerance.

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40 TRIPLES      30 PREDICATES      40 URIs      21 LITERALS

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30 sg:pageEnd 619
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32 sg:publicationDate 2009-03-05
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35 sg:scigraphId da41155eaafe233af7ecbcd773dbea7c
36 sg:title Generation of selectable marker-free transgenic tomato resistant to drought, cold and oxidative stress using the Cre/loxP DNA excision system
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40 rdfs:label Article: Generation of selectable marker-free transgenic tomato resistant to drought, cold and oxidative stress using the Cre/loxP DNA excision system
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