PUBLICATION DATE

2007-01-16

AUTHORS

Zhu-Mei He, Yu Qi, Shou-Yi Yu, Zhi-Qiang Peng, Qing Chen, Xiao-Ling Jiang

TITLE

Cholera toxin B protein in transgenic tomato fruit induces systemic immune response in mice

ISSUE

2

VOLUME

16

ISSN (print)

0962-8819

ISSN (electronic)

1573-9368

ABSTRACT

Cholera toxin B (CTB) subunit is a well-characterized antigen against cholera. Transgenic plants can offer an inexpensive and safe source of edible CTB vaccine and may be one of the best candidates for the production of plant vaccines. The present study aimed to develop transgenic tomato expressing CTB protein, especially in the ripening tomato fruit under the control of the tomato fruit-specific E8 promoter by using Agrobacterium-mediated transformation. Transgenic plants were selected using PCR and Southern blot analysis. Exogenous protein extracted from leaf, stem, and fruit tissues of transgenic plants was detected by ELISA and Western blot analysis, showing specific expression in the ripening fruit, with the highest amount of CTB protein being 0.081% of total soluble protein. Gavage of mice with ripe transgenic tomato fruits induced both serum and mucosal CTB specific antibodies. These results demonstrate the immunogenicity of the CTB protein in transgenic tomato and provide a considerable basis for exploring the utilization of CTB in the development of tomato-based edible vaccine against cholera. The rCTB antigen resulted in much lower antibody titers than an equal amount of exgenous CTB in trangenic fruits, suggesting the protective effect of the fibrous tissue of the fruit to the exogenous CTB protein against the degradation of protease in the digestive tracts of mice.

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1 articles:d6e06b046a10fc8defe9d5affad8ddda sg:abstract Abstract Cholera toxin B (CTB) subunit is a well-characterized antigen against cholera. Transgenic plants can offer an inexpensive and safe source of edible CTB vaccine and may be one of the best candidates for the production of plant vaccines. The present study aimed to develop transgenic tomato expressing CTB protein, especially in the ripening tomato fruit under the control of the tomato fruit-specific E8 promoter by using Agrobacterium-mediated transformation. Transgenic plants were selected using PCR and Southern blot analysis. Exogenous protein extracted from leaf, stem, and fruit tissues of transgenic plants was detected by ELISA and Western blot analysis, showing specific expression in the ripening fruit, with the highest amount of CTB protein being 0.081% of total soluble protein. Gavage of mice with ripe transgenic tomato fruits induced both serum and mucosal CTB specific antibodies. These results demonstrate the immunogenicity of the CTB protein in transgenic tomato and provide a considerable basis for exploring the utilization of CTB in the development of tomato-based edible vaccine against cholera. The rCTB antigen resulted in much lower antibody titers than an equal amount of exgenous CTB in trangenic fruits, suggesting the protective effect of the fibrous tissue of the fruit to the exogenous CTB protein against the degradation of protease in the digestive tracts of mice.
2 sg:articleType OriginalPaper
3 sg:coverYear 2007
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27 sg:language English
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29 sg:pageEnd 175
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31 sg:publicationDate 2007-01-16
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34 sg:scigraphId d6e06b046a10fc8defe9d5affad8ddda
35 sg:title Cholera toxin B protein in transgenic tomato fruit induces systemic immune response in mice
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37 sg:webpage https://link.springer.com/10.1007/s11248-006-9023-5
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39 rdfs:label Article: Cholera toxin B protein in transgenic tomato fruit induces systemic immune response in mice
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