PUBLICATION DATE

2016-10-27

AUTHORS

Rahul Kumar, Amit Pareek, Arun K. Sharma, Priyanka Agarwal

TITLE

Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis

ISSUE

1

VOLUME

292

ISSN (print)

1617-4615

ISSN (electronic)

1617-4623

ABSTRACT

Isolation and functional characterization of tissue- and stage-specific gene promoters is beneficial for genetic improvement of economically important crops. Here, we have characterized a putative promoter of a ripening-induced gene RIP1 (Ripening induced protein 1) in tomato. Quantification of the transcript level of RIP1 showed that its expression is fruit preferential, with maximum accumulation in red ripe fruits. To test the promoter activity, we made a reporter construct by cloning 1450 bp putative RIP1 promoter driving the GUS (ß-glucuronidase) gene expression and generated stable transgenic lines in tomato and Arabidopsis. Histochemical and fluorometric assays validated the fruit-specific expression of RIP1 as the highest GUS activity was found in red ripe tomatoes. Similarly, we detected high levels of GUS activity in the siliques of Arabidopsis. On the contrary, weak GUS activity was found in the flower buds in both tomato and Arabidopsis. To characterize the specific regions of the RIP1 promoter that might be essential for its maximum activity and specificity in fruits, we made stable transgenic lines of tomato and Arabidopsis with 5′-deletion constructs. Characterization of these transgenic plants showed that the full length promoter is essential for its function. Overall, we report the identification and characterization of a ripening-induced promoter of tomato, which would be useful for the controlled manipulation of the ripening-related agronomic traits in genetic manipulation studies in future.

How to use: Click on a object to move its position. Double click to open its homepage. Right click to preview its contents.

Download the RDF metadata as:   json-ld nt turtle xml License info


38 TRIPLES      30 PREDICATES      39 URIs      22 LITERALS

Subject Predicate Object
1 articles:d4ec3a89bd2d7931452ef441f1a0aae3 sg:abstract Abstract Isolation and functional characterization of tissue- and stage-specific gene promoters is beneficial for genetic improvement of economically important crops. Here, we have characterized a putative promoter of a ripening-induced gene RIP1 (Ripening induced protein 1) in tomato. Quantification of the transcript level of RIP1 showed that its expression is fruit preferential, with maximum accumulation in red ripe fruits. To test the promoter activity, we made a reporter construct by cloning 1450 bp putative RIP1 promoter driving the GUS (ß-glucuronidase) gene expression and generated stable transgenic lines in tomato and Arabidopsis. Histochemical and fluorometric assays validated the fruit-specific expression of RIP1 as the highest GUS activity was found in red ripe tomatoes. Similarly, we detected high levels of GUS activity in the siliques of Arabidopsis. On the contrary, weak GUS activity was found in the flower buds in both tomato and Arabidopsis. To characterize the specific regions of the RIP1 promoter that might be essential for its maximum activity and specificity in fruits, we made stable transgenic lines of tomato and Arabidopsis with 5′-deletion constructs. Characterization of these transgenic plants showed that the full length promoter is essential for its function. Overall, we report the identification and characterization of a ripening-induced promoter of tomato, which would be useful for the controlled manipulation of the ripening-related agronomic traits in genetic manipulation studies in future.
2 sg:articleType OriginalPaper
3 sg:coverYear 2017
4 sg:coverYearMonth 2017-02
5 sg:ddsId s00438-016-1262-4
6 sg:ddsIdJournalBrand 438
7 sg:doi 10.1007/s00438-016-1262-4
8 sg:doiLink http://dx.doi.org/10.1007/s00438-016-1262-4
9 sg:hasContributingOrganization grid-institutes:grid.18048.35
10 grid-institutes:grid.8195.5
11 sg:hasContribution contributions:0c4ca36cd604d941b3c558b9cdea00fa
12 contributions:8e8a327058193559bf8c30c73b220edd
13 contributions:a2259825df209b53cd58e4592b85bd63
14 contributions:b3cb5723f0886b2f4651abf632352aed
15 sg:hasFieldOfResearchCode anzsrc-for:06
16 anzsrc-for:0604
17 anzsrc-for:0607
18 sg:hasJournal journals:a8edc7698123f861fa1de60c2949808d
19 journals:cfe9ab827c989fbddc1a737f1da2f99d
20 sg:hasJournalBrand journal-brands:33903f1c73524e9f75df71380f7d28c9
21 sg:indexingDatabase Scopus
22 Web of Science
23 sg:issnElectronic 1617-4623
24 sg:issnPrint 1617-4615
25 sg:issue 1
26 sg:language English
27 sg:license http://scigraph.springernature.com/explorer/license/
28 sg:pageEnd 156
29 sg:pageStart 145
30 sg:publicationDate 2016-10-27
31 sg:publicationYear 2016
32 sg:publicationYearMonth 2016-10
33 sg:scigraphId d4ec3a89bd2d7931452ef441f1a0aae3
34 sg:title Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis
35 sg:volume 292
36 sg:webpage https://link.springer.com/10.1007/s00438-016-1262-4
37 rdf:type sg:Article
38 rdfs:label Article: Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis
HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular JSON format for linked data.

curl -H 'Accept: application/ld+json' 'http://scigraph.springernature.com/things/articles/d4ec3a89bd2d7931452ef441f1a0aae3'

N-Triples is a line-based linked data format ideal for batch operations .

curl -H 'Accept: application/n-triples' 'http://scigraph.springernature.com/things/articles/d4ec3a89bd2d7931452ef441f1a0aae3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'http://scigraph.springernature.com/things/articles/d4ec3a89bd2d7931452ef441f1a0aae3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'http://scigraph.springernature.com/things/articles/d4ec3a89bd2d7931452ef441f1a0aae3'






Preview window. Press ESC to close (or click here)


...