PUBLICATION DATE

2011-02-27

AUTHORS

You-Wang Kim, Kyoko Hiwasa-Tanase, Hiroshi Ezura, Kazuhisa Kato, Tadayoshi Hirai

TITLE

Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter

ISSUE

6

VOLUME

20

ISSN (print)

0962-8819

ISSN (electronic)

1573-9368

ABSTRACT

The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.

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Subject Predicate Object
1 articles:cbb574ed553db348708709b45d91c9b8 sg:abstract Abstract The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.
2 sg:articleType OriginalPaper
3 sg:coverYear 2011
4 sg:coverYearMonth 2011-12
5 sg:ddsId s11248-011-9495-9
6 sg:ddsIdJournalBrand 11248
7 sg:doi 10.1007/s11248-011-9495-9
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22 sg:issnElectronic 1573-9368
23 sg:issnPrint 0962-8819
24 sg:issue 6
25 sg:language English
26 sg:license http://scigraph.springernature.com/explorer/license/
27 sg:pageEnd 1292
28 sg:pageStart 1285
29 sg:publicationDate 2011-02-27
30 sg:publicationYear 2011
31 sg:publicationYearMonth 2011-02
32 sg:scigraphId cbb574ed553db348708709b45d91c9b8
33 sg:title Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter
34 sg:volume 20
35 sg:webpage https://link.springer.com/10.1007/s11248-011-9495-9
36 rdf:type sg:Article
37 rdfs:label Article: Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter
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