PUBLICATION DATE

2012-01-05

AUTHORS

A. A. Zhuchenko, V. V. Komakhina, N. A. Milyukova, R. A. Komakhin

TITLE

Analysis of the meiotic recombination frequency in transgenic tomato hybrids expressing recA and NLS-recA-licBM3 genes

ISSUE

1

VOLUME

48

ISSN (print)

1022-7954

ISSN (electronic)

1608-3369

ABSTRACT

To study and induce meiotic recombination in plants, we generated and analyzed transgenic tomato hybrids F1-RecA and F1-NLS-recA-LicBM3 expressing, respectively, the recA gene of Escherichia coli and the NLS-recA-licBM3 gene. It was found that the recA and NLS-recA-licBM3 genes are inherited through the maternal and paternal lineages, they have no selective influence on the pollen and are contained in tomato F1-RecA and F1-NLS-RecA-LicBM3 hybrids outside the second chromosome in the hemizygous state. The comparative analysis of the meiotic recombination frequency (rf) in the progenies of the transgenic and nontransgenic hybrids showed that only the expression of the recA gene of E. coli in cells of the F1-RecA plants produced a 1.2–1.5-fold increase in the frequency of recombination between some linked marker genes of the second chromosome of tomato.

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38 TRIPLES      30 PREDICATES      37 URIs      20 LITERALS

Subject Predicate Object
1 articles:c6accc63eb27ed33e51dd47708e2149d sg:abstract Abstract To study and induce meiotic recombination in plants, we generated and analyzed transgenic tomato hybrids F1-RecA and F1-NLS-recA-LicBM3 expressing, respectively, the recA gene of Escherichia coli and the NLS-recA-licBM3 gene. It was found that the recA and NLS-recA-licBM3 genes are inherited through the maternal and paternal lineages, they have no selective influence on the pollen and are contained in tomato F1-RecA and F1-NLS-RecA-LicBM3 hybrids outside the second chromosome in the hemizygous state. The comparative analysis of the meiotic recombination frequency (rf) in the progenies of the transgenic and nontransgenic hybrids showed that only the expression of the recA gene of E. coli in cells of the F1-RecA plants produced a 1.2–1.5-fold increase in the frequency of recombination between some linked marker genes of the second chromosome of tomato.
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7 sg:doi 10.1134/S1022795411110093
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26 sg:language English
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28 sg:pageEnd 31
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30 sg:publicationDate 2012-01-05
31 sg:publicationYear 2012
32 sg:publicationYearMonth 2012-01
33 sg:scigraphId c6accc63eb27ed33e51dd47708e2149d
34 sg:title Analysis of the meiotic recombination frequency in transgenic tomato hybrids expressing recA and NLS-recA-licBM3 genes
35 sg:volume 48
36 sg:webpage https://link.springer.com/10.1134/S1022795411110093
37 rdf:type sg:Article
38 rdfs:label Article: Analysis of the meiotic recombination frequency in transgenic tomato hybrids expressing recA and NLS-recA-licBM3 genes
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