PUBLICATION DATE

1997-07

AUTHORS

James W. Demski, Robert L. Jarret, Zhijian Li

TITLE

Engineered resistance to tomato spotted wilt virus in transgenic peanut expressing the viral nucleocapsid gene

ISSUE

4

VOLUME

6

ISSN (print)

0962-8819

ISSN (electronic)

1573-9368

ABSTRACT

The nucleocapsid gene of tomato spotted wilt virus Hawaiian L isolate in a sense orientation, and the GUS and NPTII marker genes, were introduced into peanut (Arachis hypogaea cv. New Mexico Valencia A) using Agrobacterium-mediated transformation. Modifications to a previously defined transformation protocol reduced the time required for production of transformed peanut plants. Transgenes were stably integrated into the peanut genome and transmitted to progeny. RNA expression and production of nucleocapsid protein in transgenic peanut were observed. Progeny of transgenic peanut plants expressing the nucleocapsid gene showed a 10- to 15-day delay in symptom development after mechanical inoculations with the donor isolate of tomato spotted wilt virus. All transgenic plants were protected from systemic tomato spotted wilt virus infection. Inoculated non-transformed control plants and plants transformed with a gene cassette not containing the nucleocapsid gene became systemically infected and displayed typical tomato spotted wilt virus symptoms. These results demonstrate that protection against tomato spotted wilt virus can be achieved in transgenic peanut plants by expression of the sense RNA of the tomato spotted wilt virus nucleocapsid gene

Related objects

How to use: Click on a object to move its position. Double click to open its homepage. Right click to preview its contents.

Download the RDF metadata as:   json-ld nt turtle xml License info


35 TRIPLES      29 PREDICATES      34 URIs      18 LITERALS

Subject Predicate Object
1 articles:9aeeffebbcd0c1b3c1a67b01e7a44477 sg:abstract Abstract The nucleocapsid gene of tomato spotted wilt virus Hawaiian L isolate in a sense orientation, and the GUS and NPTII marker genes, were introduced into peanut (Arachis hypogaea cv. New Mexico Valencia A) using Agrobacterium-mediated transformation. Modifications to a previously defined transformation protocol reduced the time required for production of transformed peanut plants. Transgenes were stably integrated into the peanut genome and transmitted to progeny. RNA expression and production of nucleocapsid protein in transgenic peanut were observed. Progeny of transgenic peanut plants expressing the nucleocapsid gene showed a 10- to 15-day delay in symptom development after mechanical inoculations with the donor isolate of tomato spotted wilt virus. All transgenic plants were protected from systemic tomato spotted wilt virus infection. Inoculated non-transformed control plants and plants transformed with a gene cassette not containing the nucleocapsid gene became systemically infected and displayed typical tomato spotted wilt virus symptoms. These results demonstrate that protection against tomato spotted wilt virus can be achieved in transgenic peanut plants by expression of the sense RNA of the tomato spotted wilt virus nucleocapsid gene
2 sg:articleType OriginalPaper
3 sg:coverYear 1997
4 sg:coverYearMonth 1997-07
5 sg:ddsId Art6
6 sg:ddsIdJournalBrand 11248
7 sg:doi 10.1023/A:1018462729127
8 sg:doiLink http://dx.doi.org/10.1023/A:1018462729127
9 sg:hasContributingOrganization grid-institutes:grid.213876.9
10 grid-institutes:grid.463419.d
11 sg:hasContribution contributions:851091d5a52f6b99f8ce7be6b728d4af
12 contributions:bc5917d5c94510b9f52262eb34ea7abd
13 contributions:f5105d924e04a91b9e23ae70e36e1b62
14 sg:hasFieldOfResearchCode anzsrc-for:06
15 anzsrc-for:0604
16 anzsrc-for:0607
17 sg:hasJournal journals:40624f5c365a8925329deb1eef71ccb3
18 journals:4bba2e67ec858431d63d1d0e1d54ae60
19 sg:hasJournalBrand journal-brands:a0cb41270a26caca02f3e80cb1121e77
20 sg:indexingDatabase Web of Science
21 sg:issnElectronic 1573-9368
22 sg:issnPrint 0962-8819
23 sg:issue 4
24 sg:language English
25 sg:license http://scigraph.springernature.com/explorer/license/
26 sg:pageEnd 305
27 sg:pageStart 297
28 sg:publicationYear 1997
29 sg:publicationYearMonth 1997-07
30 sg:scigraphId 9aeeffebbcd0c1b3c1a67b01e7a44477
31 sg:title Engineered resistance to tomato spotted wilt virus in transgenic peanut expressing the viral nucleocapsid gene
32 sg:volume 6
33 sg:webpage https://link.springer.com/10.1023/A:1018462729127
34 rdf:type sg:Article
35 rdfs:label Article: Engineered resistance to tomato spotted wilt virus in transgenic peanut expressing the viral nucleocapsid gene
HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular JSON format for linked data.

curl -H 'Accept: application/ld+json' 'http://scigraph.springernature.com/things/articles/9aeeffebbcd0c1b3c1a67b01e7a44477'

N-Triples is a line-based linked data format ideal for batch operations .

curl -H 'Accept: application/n-triples' 'http://scigraph.springernature.com/things/articles/9aeeffebbcd0c1b3c1a67b01e7a44477'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'http://scigraph.springernature.com/things/articles/9aeeffebbcd0c1b3c1a67b01e7a44477'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'http://scigraph.springernature.com/things/articles/9aeeffebbcd0c1b3c1a67b01e7a44477'






Preview window. Press ESC to close (or click here)


...