PUBLICATION DATE

1989-07

AUTHORS

Michael W. Lassner, Joseph M. Palys, John I. Yoder

TITLE

Genetic transactivation of Dissociation elements in transgenic tomato plants

ISSUE

1

VOLUME

218

ISSN (print)

0026-8925

ISSN (electronic)

1432-1874

ABSTRACT

Summary We have previously reported on the behaviour of the maize transposable elements Activator (Ac) and Dissociation (Ds) in transgenic tomato plants. To continue our study of the Ac-Ds family in tomato, we have constructed two Ds elements and a stable Ac derivative designated Transposase (Ts101). The two constructed Ds elements each contain a bacterial β-galactosidase gene to facilitate cloning the elements from the tomato genome by marker rescue. In Ds202, the central 1.6 kb HindIII fragment of Ac was replaced by a chimaeric bacterial β-galactosidase gene. Ds204 contains the β-galactosidase fragment from pUC19 flanked by 185 bp and 131 pb of the 5′ and 3′ termini (relative to the direction of transcription) of Ac. The Ts101 element was constructed by deleting about 50 nucleotides, including the inverted repeat, from the 3′ end of Ac. Ts101 and the two Ds elements were stable in transformed tomato plants. Transgenic plants containing either one of the two constructed Ds elements of Ds1, an element previously cloned from the maize Adh1 locus, were crossed to transgenic plants containing either Ts101 or an intact Ac element. Southern hybridization analysis showed that Ds1 and Ds202 excised from their resident T-DNA locations in all progeny which contained either Ts101 or Ac, but were stable in siblings which had not inherited a transposase. While Southern analysis did not identify transposed Ds elements in the F1 progeny, analysis of an F2 population indicated that the Ds elements had reintegrated in the tomato genome. In contrast to these results, no Ac-catalyzed excision of Ds204 was detected, even though the element contained the terminal regions of Ac capable of forming secondary structure. We have shown that Ds elements can be transactivated in transgenic tomato plants by crossing to plants containing an Ac or Ts element.

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1 articles:90d4c1f53123d1a1fa87bf1f77d86b9f sg:abstract Summary We have previously reported on the behaviour of the maize transposable elements Activator (Ac) and Dissociation (Ds) in transgenic tomato plants. To continue our study of the Ac-Ds family in tomato, we have constructed two Ds elements and a stable Ac derivative designated Transposase (Ts101). The two constructed Ds elements each contain a bacterial β-galactosidase gene to facilitate cloning the elements from the tomato genome by marker rescue. In Ds202, the central 1.6 kb HindIII fragment of Ac was replaced by a chimaeric bacterial β-galactosidase gene. Ds204 contains the β-galactosidase fragment from pUC19 flanked by 185 bp and 131 pb of the 5′ and 3′ termini (relative to the direction of transcription) of Ac. The Ts101 element was constructed by deleting about 50 nucleotides, including the inverted repeat, from the 3′ end of Ac. Ts101 and the two Ds elements were stable in transformed tomato plants. Transgenic plants containing either one of the two constructed Ds elements of Ds1, an element previously cloned from the maize Adh1 locus, were crossed to transgenic plants containing either Ts101 or an intact Ac element. Southern hybridization analysis showed that Ds1 and Ds202 excised from their resident T-DNA locations in all progeny which contained either Ts101 or Ac, but were stable in siblings which had not inherited a transposase. While Southern analysis did not identify transposed Ds elements in the F1 progeny, analysis of an F2 population indicated that the Ds elements had reintegrated in the tomato genome. In contrast to these results, no Ac-catalyzed excision of Ds204 was detected, even though the element contained the terminal regions of Ac capable of forming secondary structure. We have shown that Ds elements can be transactivated in transgenic tomato plants by crossing to plants containing an Ac or Ts element.
2 sg:articleType OriginalPaper
3 sg:coverYear 1989
4 sg:coverYearMonth 1989-07
5 sg:ddsId Art4
6 sg:ddsIdJournalBrand 438
7 sg:doi 10.1007/BF00330561
8 sg:doiLink http://dx.doi.org/10.1007/BF00330561
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18 sg:indexingDatabase Scopus
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20 sg:issnElectronic 1432-1874
21 sg:issnPrint 0026-8925
22 sg:issue 1
23 sg:language English
24 sg:license http://scigraph.springernature.com/explorer/license/
25 sg:pageEnd 32
26 sg:pageStart 25
27 sg:publicationYear 1989
28 sg:publicationYearMonth 1989-07
29 sg:scigraphId 90d4c1f53123d1a1fa87bf1f77d86b9f
30 sg:title Genetic transactivation of Dissociation elements in transgenic tomato plants
31 sg:volume 218
32 sg:webpage https://link.springer.com/10.1007/BF00330561
33 rdf:type sg:Article
34 rdfs:label Article: Genetic transactivation of Dissociation elements in transgenic tomato plants
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