PUBLICATION DATE

2015-10-02

AUTHORS

Takashi Yaeno, Sumyya Waliullah, Md. Emran Ali, Masamichi Nishiguchi, Naoto Yamaoka, Kappei Kobayashi

TITLE

Transmission of RNA silencing signal through grafting confers virus resistance from transgenically silenced tobacco rootstocks to non-transgenic tomato and tobacco scions

ISSUE

3

VOLUME

25

ISSN (print)

0971-7811

ISSN (electronic)

0974-1275

ABSTRACT

We examined the transmission of RNA silencing signal in non-transgenic tomato and tobacco scions grafted onto the tobacco Sd1 rootstocks, which is silenced in both NtTOM1 and NtTOM3 required for tobamovirus multiplication. When the non-transgenic tomato scions were grafted onto the Sd1 rootstocks, RT-PCR analysis of the scions showed the reduced level of mRNA compared with that before grafting in both LeTH3 and LeTH1, tomato homologs of NtTOM1 and NtTOM3, respectively. siRNAs from both genes were detected in the scions after grafting but not before grafting. Further tomato scions were inoculated with Tomato mosaic virus (ToMV) and used for virus infection. They showed very low level of virus accumulation. Necrotic responding tobacco to tobamovirus was grafted onto the rootstock of Sdl. RT-PCR analysis showed low level expression of both NtTOM1 and NtTOM3 in the scions but siRNA was detected after grafting. When the leaves of scions were inoculated with ToMV or Tobacco mosaic virus, they produced very few local necrotic lesions (LNLs) while the control scions did many LNLs. These results suggest that RNA silencing was transmitted to non-transgenic tomato and tobacco scions after grafting onto the Sd1 rootstocks and that virus resistance was induced in the scions.

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Subject Predicate Object
1 articles:52733d8a7396ae56c68b829fd86bc4d6 sg:abstract Abstract We examined the transmission of RNA silencing signal in non-transgenic tomato and tobacco scions grafted onto the tobacco Sd1 rootstocks, which is silenced in both NtTOM1 and NtTOM3 required for tobamovirus multiplication. When the non-transgenic tomato scions were grafted onto the Sd1 rootstocks, RT-PCR analysis of the scions showed the reduced level of mRNA compared with that before grafting in both LeTH3 and LeTH1, tomato homologs of NtTOM1 and NtTOM3, respectively. siRNAs from both genes were detected in the scions after grafting but not before grafting. Further tomato scions were inoculated with Tomato mosaic virus (ToMV) and used for virus infection. They showed very low level of virus accumulation. Necrotic responding tobacco to tobamovirus was grafted onto the rootstock of Sdl. RT-PCR analysis showed low level expression of both NtTOM1 and NtTOM3 in the scions but siRNA was detected after grafting. When the leaves of scions were inoculated with ToMV or Tobacco mosaic virus, they produced very few local necrotic lesions (LNLs) while the control scions did many LNLs. These results suggest that RNA silencing was transmitted to non-transgenic tomato and tobacco scions after grafting onto the Sd1 rootstocks and that virus resistance was induced in the scions.
2 sg:articleType OriginalPaper
3 sg:coverYear 2016
4 sg:coverYearMonth 2016-07
5 sg:ddsId s13562-015-0334-6
6 sg:ddsIdJournalBrand 13562
7 sg:doi 10.1007/s13562-015-0334-6
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22 sg:indexingDatabase Scopus
23 Web of Science
24 sg:issnElectronic 0974-1275
25 sg:issnPrint 0971-7811
26 sg:issue 3
27 sg:language English
28 sg:license http://scigraph.springernature.com/explorer/license/
29 sg:pageEnd 252
30 sg:pageStart 245
31 sg:publicationDate 2015-10-02
32 sg:publicationYear 2015
33 sg:publicationYearMonth 2015-10
34 sg:scigraphId 52733d8a7396ae56c68b829fd86bc4d6
35 sg:title Transmission of RNA silencing signal through grafting confers virus resistance from transgenically silenced tobacco rootstocks to non-transgenic tomato and tobacco scions
36 sg:volume 25
37 sg:webpage https://link.springer.com/10.1007/s13562-015-0334-6
38 rdf:type sg:Article
39 rdfs:label Article: Transmission of RNA silencing signal through grafting confers virus resistance from transgenically silenced tobacco rootstocks to non-transgenic tomato and tobacco scions
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