PUBLICATION DATE

2003-09

AUTHORS

Tae-Ho Kwon, Jae-Hwa Lee, Moon-Sik Yang, Young-Sook Kim

TITLE

Production and secretion of biologically active human granulocyte-macrophage colony stimulating factor in transgenic tomato suspension cultures

ISSUE

18

VOLUME

25

ISSN (print)

0141-5492

ISSN (electronic)

1573-6776

ABSTRACT

A complementary DNA encoding human granulocyte-macrophage colony stimulating factor (hGM-CSF) was cloned and introduced into tomato (Lycopersicon esculentum cv. Seokwang) using Agrobacterium-mediated transformation. Genomic PCR and Northern blot analysis demonstrated the integration of the construction into the plant nuclear genome and expression of the hGM-CSF in transgenic tomato. The cell suspension culture was established from leaf-derived calli of the transgenic tomato plants transformed with the hGM-CSF gene. Recombinant hGM-CSF was synthesized by the transgenic cell culture and secreted into the growth medium at 45 μg l−1 after 10 d' cultivation.

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7 sg:doi 10.1023/A:1025409927790
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25 sg:language English
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27 sg:pageEnd 1574
28 sg:pageStart 1571
29 sg:publicationYear 2003
30 sg:publicationYearMonth 2003-09
31 sg:scigraphId 489c522e557c422b639c653c65951d96
32 sg:title Production and secretion of biologically active human granulocyte-macrophage colony stimulating factor in transgenic tomato suspension cultures
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34 sg:webpage https://link.springer.com/10.1023/A:1025409927790
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36 rdfs:label Article: Production and secretion of biologically active human granulocyte-macrophage colony stimulating factor in transgenic tomato suspension cultures
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