PUBLICATION DATE

1997-11

AUTHORS

Clarence A. Ryan, Barry McGurl, John Delano-Freier, Vincent Franceschi, Tania Jacinto

TITLE

Tomato prosystemin promoter confers wound-inducible, vascular bundle-specific expression of the β-glucuronidase gene in transgenic tomato plants

ISSUE

4

VOLUME

203

ISSN (print)

0032-0935

ISSN (electronic)

1432-2048

ABSTRACT

. Tomato (Lycopersicon esculentum Mill. cv. Better Boy) plants were transformed with a fused gene containing a 2.2-kb promoter fragment of the tomato prosystemin gene and the coding region of the β-glucuronidase (GUS) reporter gene. The transgenic plants exhibited a low constitutive level of prosystemin-β-glucuronidase gene expression, assayed by histochemical staining and GUS enzyme activity, that was associated in the vascular bundles of leaf main veins, petiolules, petioles and stems. The GUS activity in the vascular bundles in each tissue was increased by wounding and by treatment of the plants with methyl jasmonate, similar to the induction of prosystemin in wild-type plants. The increase in GUS activity in the vascular bundles of leaves in response to wounding correlated with the wound-inducible increase in prosystemin mRNA. Tissue printing, using rabbit anti-serum prepared against prosystemin, confirmed that inducible prosystemin protein was localized in vascular bundles of petiolules, petioles and stems of wild-type tomato plants. The evidence indicates that the 2.2-kb promoter region of the tomato prosystemin gene contains elements conferring its correct temporal and spatial expression in the vascular bundles of transgenic tomato plants.

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ORGANIZATION

  • Washington State University

  • JOURNAL BRAND

  • Planta

  • FIELD OF RESEARCH CODES

  • Biological Sciences
  • Plant Biology

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    35 TRIPLES      29 PREDICATES      34 URIs      18 LITERALS

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    2 sg:articleType OriginalPaper
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    7 sg:doi 10.1007/s004250050207
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    24 sg:language English
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    26 sg:pageEnd 412
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    28 sg:publicationYear 1997
    29 sg:publicationYearMonth 1997-11
    30 sg:scigraphId 316a7bb25caac09b51351cec4d4b1f55
    31 sg:title Tomato prosystemin promoter confers wound-inducible, vascular bundle-specific expression of the β-glucuronidase gene in transgenic tomato plants
    32 sg:volume 203
    33 sg:webpage https://link.springer.com/10.1007/s004250050207
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    35 rdfs:label Article: Tomato prosystemin promoter confers wound-inducible, vascular bundle-specific expression of the β-glucuronidase gene in transgenic tomato plants
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