PUBLICATION DATE

1993-01

AUTHORS

E. Galun, R. Perl-Treves, A. Perl, S. Malkin, D. Aviv, S. Galili, E. Shalgi

TITLE

Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases

ISSUE

5

VOLUME

85

ISSN (print)

0040-5752

ISSN (electronic)

1432-2242

ABSTRACT

Summary The two cDNAs coding for the cytosolic (cyt) and the chloroplast-located (chl) Cu,Zn superoxide dismutases (SODs) of tomato (Perl-Treves et al. 1988) were cloned into respective binary vectors and mobilized into Agrobacterium strains. Potato tuber discs were infected with either of the two agrobacterial strains and cultured on selective medium containing kanaymcin. The integration of either of the cyt or the chl SOD transgenes was verified by Southern-blot hybridization. The enzymatic activity of the additional tomato chl Cu,Zn SOD could be distinguished from endogenous SOD activity since the latter isozyme migrated faster on SOD-activity gels. Several transgenic potato lines harboring either the cyt or the chl SOD genes of tomato showed elevated tolerance to the superoxide-generating herbicide paraquat (methyl viologen). After exposure of shoots to paraquat, tolerance was recorded either by scoring symptoms visually or by measurements of photosynthesis using the photoacoustic method. Root cultures from transgenic lines that harbored the additional cyt Cu,Zn SOD gene of tomato were tolerant to methyl viologen up to 10-5 M; a lower tolerance was recorded in roots of transgenic lines that expressed the additional chl Cu,Zn SOD of tomato.

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36 TRIPLES      28 PREDICATES      35 URIs      17 LITERALS

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1 articles:3078593d1000b42d509337b562e0e543 sg:abstract Summary The two cDNAs coding for the cytosolic (cyt) and the chloroplast-located (chl) Cu,Zn superoxide dismutases (SODs) of tomato (Perl-Treves et al. 1988) were cloned into respective binary vectors and mobilized into Agrobacterium strains. Potato tuber discs were infected with either of the two agrobacterial strains and cultured on selective medium containing kanaymcin. The integration of either of the cyt or the chl SOD transgenes was verified by Southern-blot hybridization. The enzymatic activity of the additional tomato chl Cu,Zn SOD could be distinguished from endogenous SOD activity since the latter isozyme migrated faster on SOD-activity gels. Several transgenic potato lines harboring either the cyt or the chl SOD genes of tomato showed elevated tolerance to the superoxide-generating herbicide paraquat (methyl viologen). After exposure of shoots to paraquat, tolerance was recorded either by scoring symptoms visually or by measurements of photosynthesis using the photoacoustic method. Root cultures from transgenic lines that harbored the additional cyt Cu,Zn SOD gene of tomato were tolerant to methyl viologen up to 10-5 M; a lower tolerance was recorded in roots of transgenic lines that expressed the additional chl Cu,Zn SOD of tomato.
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3 sg:coverYear 1993
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5 sg:ddsId Art11
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7 sg:doi 10.1007/BF00220915
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25 sg:language English
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27 sg:pageEnd 576
28 sg:pageStart 568
29 sg:publicationYear 1993
30 sg:publicationYearMonth 1993-01
31 sg:scigraphId 3078593d1000b42d509337b562e0e543
32 sg:title Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases
33 sg:volume 85
34 sg:webpage https://link.springer.com/10.1007/BF00220915
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36 rdfs:label Article: Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases
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