PUBLICATION DATE

2009-10-27

AUTHORS

Tzahi Arazi, Anat Hendelman, Kobi Buxdorf, Ran Stav

TITLE

Transgenic expression of tomato bushy stunt virus silencing suppressor P19 via the pOp/LhG4 transactivation system induces viral-like symptoms in tomato

ISSUE

1

VOLUME

40

ISSN (print)

0920-8569

ISSN (electronic)

1572-994X

ABSTRACT

During natural infection, the Tomato bushy stunt virus (TBSV) silencing suppressor protein P19 is expressed at high levels, which are required for optimum viral pathogenicity and silencing suppression. To date, expression of P19 in transgenic host plants has failed to achieve comparable expression levels and thus has provided only limited information on its in planta effects. To obtain high P19 expression and study its effects on host plant development in the absence of virus infection, we generated HA-tagged P19 (P19HA)-transgenic tomato reporter plants using the pOp/LhG4 transactivation system, which separates transformation from transgene expression. Upon reporter plant activation with a strong constitutive promoter, the transactivated F1 plants expressed high levels of a functional P19HA protein and displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms described previously for TBSV-infected tomato. Moreover, phenotype severity correlated with P19HA expression level, amount of bound miRNA/miRNA* duplexes, and accumulation of miRNA target transcripts. Together our results demonstrate that the tomato miRNA pathway is markedly compromised by P19, in particular when this protein is relatively abundant, as occurs during natural infection. We suggest that such interference with endogenous silencing may be responsible for at least some of the symptoms characteristic of TBSV-infected tomato.

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ORGANIZATION

  • Agricultural Research Organization

  • JOURNAL BRAND

  • Virus Genes

  • FIELD OF RESEARCH CODES

  • Biological Sciences
  • Plant Biology

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    36 TRIPLES      30 PREDICATES      37 URIs      22 LITERALS

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    1 articles:147bc202e25a04e7b389eb352f03907e sg:abstract Abstract During natural infection, the Tomato bushy stunt virus (TBSV) silencing suppressor protein P19 is expressed at high levels, which are required for optimum viral pathogenicity and silencing suppression. To date, expression of P19 in transgenic host plants has failed to achieve comparable expression levels and thus has provided only limited information on its in planta effects. To obtain high P19 expression and study its effects on host plant development in the absence of virus infection, we generated HA-tagged P19 (P19HA)-transgenic tomato reporter plants using the pOp/LhG4 transactivation system, which separates transformation from transgene expression. Upon reporter plant activation with a strong constitutive promoter, the transactivated F1 plants expressed high levels of a functional P19HA protein and displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms described previously for TBSV-infected tomato. Moreover, phenotype severity correlated with P19HA expression level, amount of bound miRNA/miRNA* duplexes, and accumulation of miRNA target transcripts. Together our results demonstrate that the tomato miRNA pathway is markedly compromised by P19, in particular when this protein is relatively abundant, as occurs during natural infection. We suggest that such interference with endogenous silencing may be responsible for at least some of the symptoms characteristic of TBSV-infected tomato.
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    24 sg:language English
    25 sg:license http://scigraph.springernature.com/explorer/license/
    26 sg:pageEnd 129
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    28 sg:publicationDate 2009-10-27
    29 sg:publicationYear 2009
    30 sg:publicationYearMonth 2009-10
    31 sg:scigraphId 147bc202e25a04e7b389eb352f03907e
    32 sg:title Transgenic expression of tomato bushy stunt virus silencing suppressor P19 via the pOp/LhG4 transactivation system induces viral-like symptoms in tomato
    33 sg:volume 40
    34 sg:webpage https://link.springer.com/10.1007/s11262-009-0415-5
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    36 rdfs:label Article: Transgenic expression of tomato bushy stunt virus silencing suppressor P19 via the pOp/LhG4 transactivation system induces viral-like symptoms in tomato
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