PUBLICATION DATE

2015-08-09

AUTHORS

Tzahi Arazi, Shanmugam Prakash, Dalia Wolf, Victor Gaba, Ghandi Anfoka, Diana Leibman, Sabrina Haviv, Amit Gal-On, Marina Brumin, Aaron Zelcer, Moshe Lapidot

TITLE

Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA

ISSUE

11

VOLUME

160

ISSN (print)

0304-8608

ISSN (electronic)

1432-8798

ABSTRACT

Gene silencing is a natural defense response of plants against invading RNA and DNA viruses. The RNA post-transcriptional silencing system has been commonly utilized to generate transgenic crop plants that are “immune” to plant virus infection. Here, we applied this approach against the devastating DNA virus tomato yellow leaf curl virus (TYLCV) in its host tomato (Solanum lycopersicum L.). To generate broad resistance to a number of different TYLCV viruses, three conserved sequences (the intergenic region [NCR], V1-V2 and C1-C2 genes) from the genome of the severe virus (TYLCV) were synthesized as a single insert and cloned into a hairpin configuration in a binary vector, which was used to transform TYLCV-susceptible tomato plants. Eight of 28 independent transgenic tomato lines exhibited immunity to TYLCV-Is and to TYLCV-Mld, but not to tomato yellow leaf curl Sardinia virus, which shares relatively low sequence homology with the transgene. In addition, a marker-free (nptII-deleted) transgenic tomato line was generated for the first time by Agrobacterium-mediated transformation without antibiotic selection, followed by screening of 1180 regenerated shoots by whitefly-mediated TYLCV inoculation. Resistant lines showed a high level of transgene-siRNA (t-siRNA) accumulation (22 % of total small RNA) with dominant sizes of 21 nt (73 %) and 22 nt (22 %). The t-siRNA displayed hot-spot distribution (“peaks”) along the transgene, with different distribution patterns than the viral-siRNA peaks observed in TYLCV-infected tomato. A grafting experiment demonstrated the mobility of 0.04 % of the t-siRNA from transgenic rootstock to non-transformed scion, even though scion resistance against TYLCV was not achieved.

How to use: Click on a object to move its position. Double click to open its homepage. Right click to preview its contents.

Download the RDF metadata as:   json-ld nt turtle xml License info


44 TRIPLES      30 PREDICATES      44 URIs      21 LITERALS

Subject Predicate Object
1 articles:13d28bddddf6d7c9cf40d836f2d17c4a sg:abstract Abstract Gene silencing is a natural defense response of plants against invading RNA and DNA viruses. The RNA post-transcriptional silencing system has been commonly utilized to generate transgenic crop plants that are “immune” to plant virus infection. Here, we applied this approach against the devastating DNA virus tomato yellow leaf curl virus (TYLCV) in its host tomato (Solanum lycopersicum L.). To generate broad resistance to a number of different TYLCV viruses, three conserved sequences (the intergenic region [NCR], V1-V2 and C1-C2 genes) from the genome of the severe virus (TYLCV) were synthesized as a single insert and cloned into a hairpin configuration in a binary vector, which was used to transform TYLCV-susceptible tomato plants. Eight of 28 independent transgenic tomato lines exhibited immunity to TYLCV-Is and to TYLCV-Mld, but not to tomato yellow leaf curl Sardinia virus, which shares relatively low sequence homology with the transgene. In addition, a marker-free (nptII-deleted) transgenic tomato line was generated for the first time by Agrobacterium-mediated transformation without antibiotic selection, followed by screening of 1180 regenerated shoots by whitefly-mediated TYLCV inoculation. Resistant lines showed a high level of transgene-siRNA (t-siRNA) accumulation (22 % of total small RNA) with dominant sizes of 21 nt (73 %) and 22 nt (22 %). The t-siRNA displayed hot-spot distribution (“peaks”) along the transgene, with different distribution patterns than the viral-siRNA peaks observed in TYLCV-infected tomato. A grafting experiment demonstrated the mobility of 0.04 % of the t-siRNA from transgenic rootstock to non-transformed scion, even though scion resistance against TYLCV was not achieved.
2 sg:articleType OriginalPaper
3 sg:coverYear 2015
4 sg:coverYearMonth 2015-11
5 sg:ddsId s00705-015-2551-7
6 sg:ddsIdJournalBrand 705
7 sg:doi 10.1007/s00705-015-2551-7
8 sg:doiLink http://dx.doi.org/10.1007/s00705-015-2551-7
9 sg:hasContributingOrganization grid-institutes:grid.410498.0
10 grid-institutes:grid.443749.9
11 sg:hasContribution contributions:3eab75e8ca71cdfee998d7a2d5947c7b
12 contributions:530a26a087e2c715400ff4b9d65bf5fd
13 contributions:82a0b30ac5f7026f65f979f32b689ed1
14 contributions:888eb2f024a722b0bbbb8fe055d18482
15 contributions:b1b7fff9ecaad1aa73215692e3b43828
16 contributions:b79bbf916937ec15da38eb491aef5c6a
17 contributions:d3eb1706fc0a3f049475eaa5b173df39
18 contributions:db4e2d18d76ba00f5cc2cd46b76371da
19 contributions:dbb88601657033a77d416423becad2a0
20 contributions:f198b1e576dfc2dd7180e0c8c6e52e48
21 contributions:f502a46da440bc2ba6988ff97c4c3821
22 sg:hasFieldOfResearchCode anzsrc-for:06
23 anzsrc-for:0607
24 sg:hasJournal journals:6a50cdedf2f932a3a62b6665dc1127cb
25 journals:cca946d31ad91da354b4837e0a0e4c28
26 sg:hasJournalBrand journal-brands:fa065a295c5a0bb95d26a3dfbebd3ae2
27 sg:indexingDatabase Scopus
28 Web of Science
29 sg:issnElectronic 1432-8798
30 sg:issnPrint 0304-8608
31 sg:issue 11
32 sg:language English
33 sg:license http://scigraph.springernature.com/explorer/license/
34 sg:pageEnd 2739
35 sg:pageStart 2727
36 sg:publicationDate 2015-08-09
37 sg:publicationYear 2015
38 sg:publicationYearMonth 2015-08
39 sg:scigraphId 13d28bddddf6d7c9cf40d836f2d17c4a
40 sg:title Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA
41 sg:volume 160
42 sg:webpage https://link.springer.com/10.1007/s00705-015-2551-7
43 rdf:type sg:Article
44 rdfs:label Article: Immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small RNA
HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular JSON format for linked data.

curl -H 'Accept: application/ld+json' 'http://scigraph.springernature.com/things/articles/13d28bddddf6d7c9cf40d836f2d17c4a'

N-Triples is a line-based linked data format ideal for batch operations .

curl -H 'Accept: application/n-triples' 'http://scigraph.springernature.com/things/articles/13d28bddddf6d7c9cf40d836f2d17c4a'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'http://scigraph.springernature.com/things/articles/13d28bddddf6d7c9cf40d836f2d17c4a'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'http://scigraph.springernature.com/things/articles/13d28bddddf6d7c9cf40d836f2d17c4a'






Preview window. Press ESC to close (or click here)


...