PUBLICATION DATE

1990-03

AUTHORS

Jong S. Lee, Russell Johnson, Clarence A. Ryan

TITLE

Regulation of expression of a wound-inducible tomato inhibitor I gene in transgenic nightshade plants

ISSUE

3

VOLUME

14

ISSN (print)

0167-4412

ISSN (electronic)

1573-5028

ABSTRACT

A wound-inducible proteinase Inhibitor I gene from tomato containing 725 bp of the 5′ region and 2.5 kbp of the 3′ region was stably incorporated into the genome of black nightshade plants (Solanum nigrum) using an Agrobacterium Ti plasmid-derived vector. Transgenic nightshade plants were selected that expressed the tomato Inhibitor I protein in leaf tissue. The leaves of the plants contained constitutive levels of the inhibitor protein of up to 60 μg/g tissue. These levels increased by a factor of about two in response to severe wounding. Only leaves and petioles exhibited the presence of the inhibitor, indicating that the gene exhibited the same tissue specificity of expression found in situ in wounded tomato leaves. Inhibitor I was extracted from leaves of wounded transformed nightshade plants and was partially purified by affinity chromatography on a chymotrypsin-Sepharose column. The affinity-purified protein was identical to the native tomato Inhibitor I in its immunological reactivity and in its inhibitory activity against chymotrypsin. The protein exhibited the same M r of 8 kDa as the native tomato Inhibitor I and its N-terminal amino acid sequence was identical to that of the native tomato inhibitor I, indicating that the protein was properly processed in nightshade plants. These expriments are the first report of the expression of a member of the wound-inducible tomato Inhibitor I gene family in transgenic plants. The results demonstrate that the gene contains elements that can be regulated in a wound-inducible, tissuespecific manner in nightshade plants.

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Subject Predicate Object
1 articles:10138f36f4ada5ba16543274e8763e9c sg:abstract Abstract A wound-inducible proteinase Inhibitor I gene from tomato containing 725 bp of the 5′ region and 2.5 kbp of the 3′ region was stably incorporated into the genome of black nightshade plants (Solanum nigrum) using an Agrobacterium Ti plasmid-derived vector. Transgenic nightshade plants were selected that expressed the tomato Inhibitor I protein in leaf tissue. The leaves of the plants contained constitutive levels of the inhibitor protein of up to 60 μg/g tissue. These levels increased by a factor of about two in response to severe wounding. Only leaves and petioles exhibited the presence of the inhibitor, indicating that the gene exhibited the same tissue specificity of expression found in situ in wounded tomato leaves. Inhibitor I was extracted from leaves of wounded transformed nightshade plants and was partially purified by affinity chromatography on a chymotrypsin-Sepharose column. The affinity-purified protein was identical to the native tomato Inhibitor I in its immunological reactivity and in its inhibitory activity against chymotrypsin. The protein exhibited the same M r of 8 kDa as the native tomato Inhibitor I and its N-terminal amino acid sequence was identical to that of the native tomato inhibitor I, indicating that the protein was properly processed in nightshade plants. These expriments are the first report of the expression of a member of the wound-inducible tomato Inhibitor I gene family in transgenic plants. The results demonstrate that the gene contains elements that can be regulated in a wound-inducible, tissuespecific manner in nightshade plants.
2 sg:articleType OriginalPaper
3 sg:coverYear 1990
4 sg:coverYearMonth 1990-03
5 sg:ddsId Art6
6 sg:ddsIdJournalBrand 11103
7 sg:doi 10.1007/BF00028771
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19 sg:indexingDatabase Web of Science
20 sg:issnElectronic 1573-5028
21 sg:issnPrint 0167-4412
22 sg:issue 3
23 sg:language English
24 sg:license http://scigraph.springernature.com/explorer/license/
25 sg:pageEnd 356
26 sg:pageStart 349
27 sg:publicationYear 1990
28 sg:publicationYearMonth 1990-03
29 sg:scigraphId 10138f36f4ada5ba16543274e8763e9c
30 sg:title Regulation of expression of a wound-inducible tomato inhibitor I gene in transgenic nightshade plants
31 sg:volume 14
32 sg:webpage https://link.springer.com/10.1007/BF00028771
33 rdf:type sg:Article
34 rdfs:label Article: Regulation of expression of a wound-inducible tomato inhibitor I gene in transgenic nightshade plants
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