In vitro induction of anterior gradient-2-specific cytotoxic T lymphocytes by dendritic cells transduced with recombinant adenoviruses as a potential therapy ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012

AUTHORS

Hyun Ju Lee, Cheol Yi Hong, Mi-Hyun Kim, Youn-Kyung Lee, Thanh-Nhan Nguyen-Pham, Byoung Chul Park, Deok-Hwan Yang, Ik-Joo Chung, Hyeoung-Joon Kim, Je-Jung Lee

ABSTRACT

Anterior gradient-2 (AGR2) promotes tumor growth, cell migration, and cellular transformation, and is one of the specific mRNA markers for circulating tumor cells in patients with gastrointestinal cancer. We investigated the feasibility of AGR2 as a potent antigen for tumor immunotherapy against colorectal cancer (CRC) cells using dendritic cells (DCs) transduced with a recombinant adenovirus harboring the AGR2 gene (AdAGR2). DCs transduced with a recombinant adenovirus encoding the AGR2 gene (AdAGR2/DCs) were characterized. These genetically-modified DCs expressed AGR2 mRNA as well as AGR2 protein at a multiplicity of infection of 1,000 without any significant alterations in DC viability and cytokine secretion (IL-10 and IL-12p70) compared with unmodified DCs as a control. In addition, AdAGR2 transduction did not impair DC maturation, but enhanced expression of HLA-DR, CD80, and CD86. AdAGR2/DCs augmented the number of IFN-γ-secreting T-cells and elicited potent AGR2-specific cytotoxic T lymphocytes capable of lysing AGR2-expressing CRC cell lines. These results suggest that AGR2 act as a potentially important antigen for immunotherapy against CRC in clinical applications. More... »

PAGES

emm20128

Identifiers

URI

http://scigraph.springernature.com/pub.10.3858/emm.2012.44.1.006

DOI

http://dx.doi.org/10.3858/emm.2012.44.1.006

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036341934

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22089087


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenoviridae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigen Presentation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Neoplasm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colorectal Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytotoxicity, Immunologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dendritic Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunotherapy, Adoptive", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interferon-gamma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphocyte Activation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes, Cytotoxic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transgenes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chonnam National University Hwasun Hospital", 
          "id": "https://www.grid.ac/institutes/grid.411602.0", 
          "name": [
            "Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Hyun Ju", 
        "id": "sg:person.01247527025.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247527025.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University Hwasun Hospital", 
          "id": "https://www.grid.ac/institutes/grid.411602.0", 
          "name": [
            "Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hong", 
        "givenName": "Cheol Yi", 
        "id": "sg:person.01315642225.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315642225.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University Hwasun Hospital", 
          "id": "https://www.grid.ac/institutes/grid.411602.0", 
          "name": [
            "Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Vaxcell-Bio Therapeutics, Hwasun 519-763, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Mi-Hyun", 
        "id": "sg:person.0707543053.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707543053.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University Hwasun Hospital", 
          "id": "https://www.grid.ac/institutes/grid.411602.0", 
          "name": [
            "Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Vaxcell-Bio Therapeutics, Hwasun 519-763, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Youn-Kyung", 
        "id": "sg:person.0755656253.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755656253.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University Hwasun Hospital", 
          "id": "https://www.grid.ac/institutes/grid.411602.0", 
          "name": [
            "Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nguyen-Pham", 
        "givenName": "Thanh-Nhan", 
        "id": "sg:person.0754223607.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754223607.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Korea Research Institute of Bioscience and Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.249967.7", 
          "name": [
            "Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Park", 
        "givenName": "Byoung Chul", 
        "id": "sg:person.01065106463.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065106463.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University Hwasun Hospital", 
          "id": "https://www.grid.ac/institutes/grid.411602.0", 
          "name": [
            "Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Deok-Hwan", 
        "id": "sg:person.0753367262.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753367262.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University Hwasun Hospital", 
          "id": "https://www.grid.ac/institutes/grid.411602.0", 
          "name": [
            "Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chung", 
        "givenName": "Ik-Joo", 
        "id": "sg:person.01023725662.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023725662.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University Hwasun Hospital", 
          "id": "https://www.grid.ac/institutes/grid.411602.0", 
          "name": [
            "Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Hyeoung-Joon", 
        "id": "sg:person.0617770714.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617770714.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chonnam National University", 
          "id": "https://www.grid.ac/institutes/grid.14005.30", 
          "name": [
            "Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.", 
            "Vaxcell-Bio Therapeutics, Hwasun 519-763, Korea.", 
            "The Brain Korea 21 Project, Center for Biomedical Human Resources, Chonnam National University, Gwangju 500-757, Korea."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Je-Jung", 
        "id": "sg:person.01204441075.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204441075.42"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0925-4773(98)00021-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000801026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cdp.2008.08.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002935640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm1295-1297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003113140", 
          "https://doi.org/10.1038/nm1295-1297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004397201", 
          "https://doi.org/10.1038/sj.gt.3301210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3301210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004397201", 
          "https://doi.org/10.1038/sj.gt.3301210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1034/j.1600-065x.2002.18816.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006544799"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1053/j.gastro.2006.04.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009165402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri1592", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010707228", 
          "https://doi.org/10.1038/nri1592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri1592", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010707228", 
          "https://doi.org/10.1038/nri1592"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(91)90325-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013179911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(91)90325-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013179911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-07-6818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015048317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0398-328", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017994921", 
          "https://doi.org/10.1038/nm0398-328"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000086770", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019395220"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000086770", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019395220"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.186.7.1177", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022624613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2005.12.056", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025892563"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.186.8.1247", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030483244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-04-3823", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032074156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcla.1860050510", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032929195"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-08-1320", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034512046"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300496", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035362518", 
          "https://doi.org/10.1038/sj.gt.3300496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300496", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035362518", 
          "https://doi.org/10.1038/sj.gt.3300496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3083.1993.tb03236.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037609706"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-5699(97)84664-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038821180"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.pcan.4500960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039140319", 
          "https://doi.org/10.1038/sj.pcan.4500960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.pcan.4500960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039140319", 
          "https://doi.org/10.1038/sj.pcan.4500960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0958-1669(99)00007-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048905210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1521-4141(199903)29:03<964::aid-immu964>3.0.co;2-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049508894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1521-4141(199903)29:03<964::aid-immu964>3.0.co;2-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049508894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/0022-1317-71-10-2425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060357350"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074549010", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.178.4.1975", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077366007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083028311", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083379164", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012", 
    "datePublishedReg": "2012-01-01", 
    "description": "Anterior gradient-2 (AGR2) promotes tumor growth, cell migration, and cellular transformation, and is one of the specific mRNA markers for circulating tumor cells in patients with gastrointestinal cancer. We investigated the feasibility of AGR2 as a potent antigen for tumor immunotherapy against colorectal cancer (CRC) cells using dendritic cells (DCs) transduced with a recombinant adenovirus harboring the AGR2 gene (AdAGR2). DCs transduced with a recombinant adenovirus encoding the AGR2 gene (AdAGR2/DCs) were characterized. These genetically-modified DCs expressed AGR2 mRNA as well as AGR2 protein at a multiplicity of infection of 1,000 without any significant alterations in DC viability and cytokine secretion (IL-10 and IL-12p70) compared with unmodified DCs as a control. In addition, AdAGR2 transduction did not impair DC maturation, but enhanced expression of HLA-DR, CD80, and CD86. AdAGR2/DCs augmented the number of IFN-\u03b3-secreting T-cells and elicited potent AGR2-specific cytotoxic T lymphocytes capable of lysing AGR2-expressing CRC cell lines. These results suggest that AGR2 act as a potentially important antigen for immunotherapy against CRC in clinical applications.", 
    "genre": "research_article", 
    "id": "sg:pub.10.3858/emm.2012.44.1.006", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.7460449", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1085098", 
        "issn": [
          "1226-3613", 
          "2092-6413"
        ], 
        "name": "Experimental & Molecular Medicine", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "44"
      }
    ], 
    "name": "In vitro induction of anterior gradient-2-specific cytotoxic T lymphocytes by dendritic cells transduced with recombinant adenoviruses as a potential therapy for colorectal cancer", 
    "pagination": "emm20128", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ad1e76cce168ea8516e6c431c610397138fd5331eeec3c834f497d64cb638b36"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22089087"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9607880"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.3858/emm.2012.44.1.006"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036341934"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.3858/emm.2012.44.1.006", 
      "https://app.dimensions.ai/details/publication/pub.1036341934"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:56", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000425.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/emm20128"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.3858/emm.2012.44.1.006'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.3858/emm.2012.44.1.006'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.3858/emm.2012.44.1.006'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.3858/emm.2012.44.1.006'


 

This table displays all metadata directly associated to this object as RDF triples.

300 TRIPLES      21 PREDICATES      74 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.3858/emm.2012.44.1.006 schema:about N23de5cb29c294bb6b878a8db4dae03b0
2 N26f078fe8d4d4d3bbc71d8948a4bd5ea
3 N3f462afb6b84410589016af0c54c6b8d
4 N5311364139654740b69e84f744ce2b6d
5 N55a2f8d05bb9425a8c6165b48f04d7e6
6 N7082bb72926d455cbf287730193ef6c7
7 N7656a13a735743d68e8f7df5509ccd46
8 N7bab592e475f4a2f94931acc1a2dc0fb
9 N860fe3c40d9d44ee8a3e5fb0c05b50b3
10 N8de3861d481f48aa983c254127ff9867
11 N95353e68598644ad8321924b6a6ac81c
12 Na3e4742473054bd697151cfa3eceb3ac
13 Nc1fa288d7d3e4575a69fd9420e8f4ff1
14 Ncedbc136245247969fde9492423b53cd
15 Nd1e227917ce1466b8db5a51e7044bb0c
16 Ndbe2ee0aa7d242f7a11ea715e43a4fe2
17 Nec82627ca4cb4892bf08e67c94567567
18 anzsrc-for:11
19 anzsrc-for:1107
20 schema:author N33d9e989951441aeb2b326d0ccc29deb
21 schema:citation sg:pub.10.1038/nm0398-328
22 sg:pub.10.1038/nm1295-1297
23 sg:pub.10.1038/nri1592
24 sg:pub.10.1038/sj.gt.3300496
25 sg:pub.10.1038/sj.gt.3301210
26 sg:pub.10.1038/sj.pcan.4500960
27 https://app.dimensions.ai/details/publication/pub.1074549010
28 https://app.dimensions.ai/details/publication/pub.1083028311
29 https://app.dimensions.ai/details/publication/pub.1083379164
30 https://doi.org/10.1002/(sici)1521-4141(199903)29:03<964::aid-immu964>3.0.co;2-p
31 https://doi.org/10.1002/jcla.1860050510
32 https://doi.org/10.1016/0022-1759(91)90325-a
33 https://doi.org/10.1016/j.cdp.2008.08.002
34 https://doi.org/10.1016/j.vaccine.2005.12.056
35 https://doi.org/10.1016/s0167-5699(97)84664-6
36 https://doi.org/10.1016/s0925-4773(98)00021-5
37 https://doi.org/10.1016/s0958-1669(99)00007-5
38 https://doi.org/10.1034/j.1600-065x.2002.18816.x
39 https://doi.org/10.1053/j.gastro.2006.04.026
40 https://doi.org/10.1084/jem.186.7.1177
41 https://doi.org/10.1084/jem.186.8.1247
42 https://doi.org/10.1099/0022-1317-71-10-2425
43 https://doi.org/10.1111/j.1365-3083.1993.tb03236.x
44 https://doi.org/10.1158/0008-5472.can-04-3823
45 https://doi.org/10.1158/0008-5472.can-07-6818
46 https://doi.org/10.1158/0008-5472.can-08-1320
47 https://doi.org/10.1159/000086770
48 https://doi.org/10.4049/jimmunol.178.4.1975
49 schema:datePublished 2012
50 schema:datePublishedReg 2012-01-01
51 schema:description Anterior gradient-2 (AGR2) promotes tumor growth, cell migration, and cellular transformation, and is one of the specific mRNA markers for circulating tumor cells in patients with gastrointestinal cancer. We investigated the feasibility of AGR2 as a potent antigen for tumor immunotherapy against colorectal cancer (CRC) cells using dendritic cells (DCs) transduced with a recombinant adenovirus harboring the AGR2 gene (AdAGR2). DCs transduced with a recombinant adenovirus encoding the AGR2 gene (AdAGR2/DCs) were characterized. These genetically-modified DCs expressed AGR2 mRNA as well as AGR2 protein at a multiplicity of infection of 1,000 without any significant alterations in DC viability and cytokine secretion (IL-10 and IL-12p70) compared with unmodified DCs as a control. In addition, AdAGR2 transduction did not impair DC maturation, but enhanced expression of HLA-DR, CD80, and CD86. AdAGR2/DCs augmented the number of IFN-γ-secreting T-cells and elicited potent AGR2-specific cytotoxic T lymphocytes capable of lysing AGR2-expressing CRC cell lines. These results suggest that AGR2 act as a potentially important antigen for immunotherapy against CRC in clinical applications.
52 schema:genre research_article
53 schema:inLanguage en
54 schema:isAccessibleForFree true
55 schema:isPartOf Naa3300f9fcf94988809600b1e48874e5
56 Nd00eb3291ff742fd94c7751a77d85f26
57 sg:journal.1085098
58 schema:name In vitro induction of anterior gradient-2-specific cytotoxic T lymphocytes by dendritic cells transduced with recombinant adenoviruses as a potential therapy for colorectal cancer
59 schema:pagination emm20128
60 schema:productId N42b5a0d41a1f4cbcbc80db8645761838
61 N7ae0fe07a5af488cbd7d96a37fa12862
62 N87a608ba3ce644b7a97bf8b1629f980f
63 Ncb49e0e114684b448bdf86f89b617375
64 Ne52e24251fb144bdac1a40f774f50a3a
65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036341934
66 https://doi.org/10.3858/emm.2012.44.1.006
67 schema:sdDatePublished 2019-04-10T13:56
68 schema:sdLicense https://scigraph.springernature.com/explorer/license/
69 schema:sdPublisher N00573f93b00842408d4062ca9015f060
70 schema:url http://www.nature.com/articles/emm20128
71 sgo:license sg:explorer/license/
72 sgo:sdDataset articles
73 rdf:type schema:ScholarlyArticle
74 N00573f93b00842408d4062ca9015f060 schema:name Springer Nature - SN SciGraph project
75 rdf:type schema:Organization
76 N0ad894c22602424ea14e34b26ce1f8f9 rdf:first sg:person.0617770714.19
77 rdf:rest Nd6deccb0098a4b99a231b38918fdf7ef
78 N23de5cb29c294bb6b878a8db4dae03b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Carcinoma
80 rdf:type schema:DefinedTerm
81 N251ee8142262437ca84e474b465e2e0c rdf:first sg:person.0753367262.83
82 rdf:rest N38a2ea2d810346b4b3d88bdbef3f8c5c
83 N26f078fe8d4d4d3bbc71d8948a4bd5ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Transgenes
85 rdf:type schema:DefinedTerm
86 N33d9e989951441aeb2b326d0ccc29deb rdf:first sg:person.01247527025.54
87 rdf:rest N5b49c2af5dd041cfa0a6439c003e8dbb
88 N38a2ea2d810346b4b3d88bdbef3f8c5c rdf:first sg:person.01023725662.02
89 rdf:rest N0ad894c22602424ea14e34b26ce1f8f9
90 N3f462afb6b84410589016af0c54c6b8d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Dendritic Cells
92 rdf:type schema:DefinedTerm
93 N42b5a0d41a1f4cbcbc80db8645761838 schema:name nlm_unique_id
94 schema:value 9607880
95 rdf:type schema:PropertyValue
96 N5311364139654740b69e84f744ce2b6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Humans
98 rdf:type schema:DefinedTerm
99 N55a2f8d05bb9425a8c6165b48f04d7e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Lymphocyte Activation
101 rdf:type schema:DefinedTerm
102 N5b49c2af5dd041cfa0a6439c003e8dbb rdf:first sg:person.01315642225.12
103 rdf:rest N8a4dfacfa25d4aa69e345d349aae2e9c
104 N6cf95863974845a8963fc537f7fba15e rdf:first sg:person.0754223607.21
105 rdf:rest Ncfeb16e3089143ccb3341a8cb675a2b6
106 N7082bb72926d455cbf287730193ef6c7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Colorectal Neoplasms
108 rdf:type schema:DefinedTerm
109 N7656a13a735743d68e8f7df5509ccd46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Biomarkers, Tumor
111 rdf:type schema:DefinedTerm
112 N7ae0fe07a5af488cbd7d96a37fa12862 schema:name pubmed_id
113 schema:value 22089087
114 rdf:type schema:PropertyValue
115 N7bab592e475f4a2f94931acc1a2dc0fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Adenoviridae
117 rdf:type schema:DefinedTerm
118 N7dda786356ce46e8b7aaa9877f14504d rdf:first sg:person.0755656253.10
119 rdf:rest N6cf95863974845a8963fc537f7fba15e
120 N860fe3c40d9d44ee8a3e5fb0c05b50b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Antigen Presentation
122 rdf:type schema:DefinedTerm
123 N87a608ba3ce644b7a97bf8b1629f980f schema:name doi
124 schema:value 10.3858/emm.2012.44.1.006
125 rdf:type schema:PropertyValue
126 N8a4dfacfa25d4aa69e345d349aae2e9c rdf:first sg:person.0707543053.63
127 rdf:rest N7dda786356ce46e8b7aaa9877f14504d
128 N8de3861d481f48aa983c254127ff9867 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Transduction, Genetic
130 rdf:type schema:DefinedTerm
131 N95353e68598644ad8321924b6a6ac81c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Antigens, Neoplasm
133 rdf:type schema:DefinedTerm
134 Na3e4742473054bd697151cfa3eceb3ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Immunotherapy, Adoptive
136 rdf:type schema:DefinedTerm
137 Naa3300f9fcf94988809600b1e48874e5 schema:volumeNumber 44
138 rdf:type schema:PublicationVolume
139 Nc1fa288d7d3e4575a69fd9420e8f4ff1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Cell Line, Tumor
141 rdf:type schema:DefinedTerm
142 Ncb49e0e114684b448bdf86f89b617375 schema:name readcube_id
143 schema:value ad1e76cce168ea8516e6c431c610397138fd5331eeec3c834f497d64cb638b36
144 rdf:type schema:PropertyValue
145 Ncedbc136245247969fde9492423b53cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name T-Lymphocytes, Cytotoxic
147 rdf:type schema:DefinedTerm
148 Ncfeb16e3089143ccb3341a8cb675a2b6 rdf:first sg:person.01065106463.41
149 rdf:rest N251ee8142262437ca84e474b465e2e0c
150 Nd00eb3291ff742fd94c7751a77d85f26 schema:issueNumber 1
151 rdf:type schema:PublicationIssue
152 Nd1e227917ce1466b8db5a51e7044bb0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Proteins
154 rdf:type schema:DefinedTerm
155 Nd6deccb0098a4b99a231b38918fdf7ef rdf:first sg:person.01204441075.42
156 rdf:rest rdf:nil
157 Ndbe2ee0aa7d242f7a11ea715e43a4fe2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Interferon-gamma
159 rdf:type schema:DefinedTerm
160 Ne52e24251fb144bdac1a40f774f50a3a schema:name dimensions_id
161 schema:value pub.1036341934
162 rdf:type schema:PropertyValue
163 Nec82627ca4cb4892bf08e67c94567567 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Cytotoxicity, Immunologic
165 rdf:type schema:DefinedTerm
166 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
167 schema:name Medical and Health Sciences
168 rdf:type schema:DefinedTerm
169 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
170 schema:name Immunology
171 rdf:type schema:DefinedTerm
172 sg:grant.7460449 http://pending.schema.org/fundedItem sg:pub.10.3858/emm.2012.44.1.006
173 rdf:type schema:MonetaryGrant
174 sg:journal.1085098 schema:issn 1226-3613
175 2092-6413
176 schema:name Experimental & Molecular Medicine
177 rdf:type schema:Periodical
178 sg:person.01023725662.02 schema:affiliation https://www.grid.ac/institutes/grid.411602.0
179 schema:familyName Chung
180 schema:givenName Ik-Joo
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023725662.02
182 rdf:type schema:Person
183 sg:person.01065106463.41 schema:affiliation https://www.grid.ac/institutes/grid.249967.7
184 schema:familyName Park
185 schema:givenName Byoung Chul
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065106463.41
187 rdf:type schema:Person
188 sg:person.01204441075.42 schema:affiliation https://www.grid.ac/institutes/grid.14005.30
189 schema:familyName Lee
190 schema:givenName Je-Jung
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204441075.42
192 rdf:type schema:Person
193 sg:person.01247527025.54 schema:affiliation https://www.grid.ac/institutes/grid.411602.0
194 schema:familyName Lee
195 schema:givenName Hyun Ju
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01247527025.54
197 rdf:type schema:Person
198 sg:person.01315642225.12 schema:affiliation https://www.grid.ac/institutes/grid.411602.0
199 schema:familyName Hong
200 schema:givenName Cheol Yi
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315642225.12
202 rdf:type schema:Person
203 sg:person.0617770714.19 schema:affiliation https://www.grid.ac/institutes/grid.411602.0
204 schema:familyName Kim
205 schema:givenName Hyeoung-Joon
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617770714.19
207 rdf:type schema:Person
208 sg:person.0707543053.63 schema:affiliation https://www.grid.ac/institutes/grid.411602.0
209 schema:familyName Kim
210 schema:givenName Mi-Hyun
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707543053.63
212 rdf:type schema:Person
213 sg:person.0753367262.83 schema:affiliation https://www.grid.ac/institutes/grid.411602.0
214 schema:familyName Yang
215 schema:givenName Deok-Hwan
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753367262.83
217 rdf:type schema:Person
218 sg:person.0754223607.21 schema:affiliation https://www.grid.ac/institutes/grid.411602.0
219 schema:familyName Nguyen-Pham
220 schema:givenName Thanh-Nhan
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754223607.21
222 rdf:type schema:Person
223 sg:person.0755656253.10 schema:affiliation https://www.grid.ac/institutes/grid.411602.0
224 schema:familyName Lee
225 schema:givenName Youn-Kyung
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755656253.10
227 rdf:type schema:Person
228 sg:pub.10.1038/nm0398-328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017994921
229 https://doi.org/10.1038/nm0398-328
230 rdf:type schema:CreativeWork
231 sg:pub.10.1038/nm1295-1297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003113140
232 https://doi.org/10.1038/nm1295-1297
233 rdf:type schema:CreativeWork
234 sg:pub.10.1038/nri1592 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010707228
235 https://doi.org/10.1038/nri1592
236 rdf:type schema:CreativeWork
237 sg:pub.10.1038/sj.gt.3300496 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035362518
238 https://doi.org/10.1038/sj.gt.3300496
239 rdf:type schema:CreativeWork
240 sg:pub.10.1038/sj.gt.3301210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004397201
241 https://doi.org/10.1038/sj.gt.3301210
242 rdf:type schema:CreativeWork
243 sg:pub.10.1038/sj.pcan.4500960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039140319
244 https://doi.org/10.1038/sj.pcan.4500960
245 rdf:type schema:CreativeWork
246 https://app.dimensions.ai/details/publication/pub.1074549010 schema:CreativeWork
247 https://app.dimensions.ai/details/publication/pub.1083028311 schema:CreativeWork
248 https://app.dimensions.ai/details/publication/pub.1083379164 schema:CreativeWork
249 https://doi.org/10.1002/(sici)1521-4141(199903)29:03<964::aid-immu964>3.0.co;2-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1049508894
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1002/jcla.1860050510 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032929195
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/0022-1759(91)90325-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1013179911
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1016/j.cdp.2008.08.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002935640
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1016/j.vaccine.2005.12.056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025892563
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1016/s0167-5699(97)84664-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038821180
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1016/s0925-4773(98)00021-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000801026
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1016/s0958-1669(99)00007-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048905210
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1034/j.1600-065x.2002.18816.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006544799
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1053/j.gastro.2006.04.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009165402
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1084/jem.186.7.1177 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022624613
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1084/jem.186.8.1247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030483244
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1099/0022-1317-71-10-2425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060357350
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1111/j.1365-3083.1993.tb03236.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037609706
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1158/0008-5472.can-04-3823 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032074156
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1158/0008-5472.can-07-6818 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015048317
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1158/0008-5472.can-08-1320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034512046
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1159/000086770 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019395220
284 rdf:type schema:CreativeWork
285 https://doi.org/10.4049/jimmunol.178.4.1975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077366007
286 rdf:type schema:CreativeWork
287 https://www.grid.ac/institutes/grid.14005.30 schema:alternateName Chonnam National University
288 schema:name Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.
289 Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.
290 The Brain Korea 21 Project, Center for Biomedical Human Resources, Chonnam National University, Gwangju 500-757, Korea.
291 Vaxcell-Bio Therapeutics, Hwasun 519-763, Korea.
292 rdf:type schema:Organization
293 https://www.grid.ac/institutes/grid.249967.7 schema:alternateName Korea Research Institute of Bioscience and Biotechnology
294 schema:name Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea.
295 rdf:type schema:Organization
296 https://www.grid.ac/institutes/grid.411602.0 schema:alternateName Chonnam National University Hwasun Hospital
297 schema:name Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.
298 Research Center for Cancer Immunotherapy, Chonnam National University Hwasun Hospital, Hwasun 519-763, Korea.
299 Vaxcell-Bio Therapeutics, Hwasun 519-763, Korea.
300 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...