Absorption and fluorescence spectra of polynucleotide complexes with ethidium bromide View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-10

AUTHORS

P. O. Vardevanyan, A. P. Antonyan, M. A. Parsadanyan, M. V. Minasyants

ABSTRACT

We study the ethidium bromide (EtBr) interaction with the poly(dA)-poly(dT) and poly[d(A-T)]-poly[d(A-T)] polynucleotides at 0.02 M ionic strength of solution and pH = 7.2. It was revealed that the EtBr binds to the poly[d(A-T)]-poly[d(A-T)] by three modes: the intercalation, semiintercalation and weak (electrostatic), while with the poly(dA)-poly(dT) by two modes: the intercalation and semi-intercalation. Moreover, EtBr binds to the poly(dA)-poly(dT) cooperatively and to the poly[d(A-T)]-poly[d(A-T)] non-cooperatively. More... »

PAGES

381-387

Identifiers

URI

http://scigraph.springernature.com/pub.10.3103/s1068337215040106

DOI

http://dx.doi.org/10.3103/s1068337215040106

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046471673


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