Production of human interferon ALPHA 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-10

AUTHORS

Y. R. Sindarovska, I. M. Gerasymenko, Y. V. Sheludko, Z. M. Olevinskaya, N. Y. Spivak, N. V. Kuchuk

ABSTRACT

Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 × 103 IU/g fresh weight (FW) with an average of 2.1 ± 0.8 × 103 IU/g FW. It proves that N. excelsior is a suitable host for Agrobacterium-mediated transient expression of genes encoding physiologically active human proteins. The transient expression conditions optimized for GFP marker protein were confirmed to be preferable for hIFN α2b. More... »

PAGES

313-316

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.3103/s0095452710050099

DOI

http://dx.doi.org/10.3103/s0095452710050099

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025483934


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