First cloning and characterization of aspartate aminotransferase from river buffalo (Bubalus bubalis) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-12

AUTHORS

Muhammad Shahid Nadeem, Naeem Rashid, Muzaffar Iqbal, Qurra-tul-Ann Afza Gardner, Muhammad Akhtar

ABSTRACT

Aspartate aminotransferase catalyzes the transfer of an amino group from L-aspartate to α-oxoglutarate. We have purified cytosolic isozyme, to apparent homogeneity, from the heart of river buffalo. In order to clone the enzyme total RNA was isolated and the cDNA encoding complete polypeptide of 413 amino acids was amplified by reverse transcriptase polymerase chain reaction. The cDNA and the deduced amino acid sequence exhibited 98.2% and 99.5% identities, respectively, with that of cow. The cDNA was overexpressed in Escherichia coli and the gene product was obtained in enzymologically active form. The recombinant enzyme was about 40% of the total cell proteins. The purified recombinant enzyme displayed specific activity, Km, temperature and pH stability values similar to that of the native enzyme. Edman degradation and electrospray ionization mass spectra showed that the recombinant enzyme consisted of three species having N-terminal sequences of MAPPSIF, APPSIF and PPSIF, although the second one was the predominant species. Conditions are also described, which in the mass spectral analysis give either the three species corresponding to the apo- or the holo-enzyme; the latter retaining pyridoxal phosphate bound to the protein. To our knowledge this is the first report on sequence determination, cloning and characterization of aspartate aminotransferase from river buffalo. More... »

PAGES

1202

Identifiers

URI

http://scigraph.springernature.com/pub.10.2478/s11756-011-0125-z

DOI

http://dx.doi.org/10.2478/s11756-011-0125-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028256283


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of the Punjab", 
          "id": "https://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shahid Nadeem", 
        "givenName": "Muhammad", 
        "id": "sg:person.01074215514.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074215514.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of the Punjab", 
          "id": "https://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rashid", 
        "givenName": "Naeem", 
        "id": "sg:person.01005647064.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005647064.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of the Punjab", 
          "id": "https://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan", 
            "Calzyme Laboratories, Inc., 3443 Miguelito Court, 93401, San Luis Obispo, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Iqbal", 
        "givenName": "Muzaffar", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of the Punjab", 
          "id": "https://www.grid.ac/institutes/grid.11173.35", 
          "name": [
            "School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Afza Gardner", 
        "givenName": "Qurra-tul-Ann", 
        "id": "sg:person.01013705746.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013705746.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Southampton", 
          "id": "https://www.grid.ac/institutes/grid.5491.9", 
          "name": [
            "School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan", 
            "School of Biological Sciences, University of Southampton, SO16 7PX, Southampton, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Akhtar", 
        "givenName": "Muhammad", 
        "id": "sg:person.015572356512.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015572356512.49"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1990.tb19204.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002959078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/bcr2154", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005518856", 
          "https://doi.org/10.1186/bcr2154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0305-0491(94)00143-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007482456"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj0990589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009462695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj0990589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009462695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00042737-200409000-00013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010207753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00042737-200409000-00013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010207753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00042737-200409000-00013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010207753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(05)80360-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013618994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1968.tb00402.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021767698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/9780470122778.ch2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024891027"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-0348-9308-4_7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031687307", 
          "https://doi.org/10.1007/978-3-0348-9308-4_7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-0348-9308-4_7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031687307", 
          "https://doi.org/10.1007/978-3-0348-9308-4_7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1570-9639(03)00081-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033423229"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(84)90333-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033880362"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(88)90329-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037885513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkq1079", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038685652"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(63)90346-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041824850"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/22.22.4673", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042438223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1010618203054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043560335", 
          "https://doi.org/10.1023/a:1010618203054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0009-8981(82)90071-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047848829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0009-8981(82)90071-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047848829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.92.3.587", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050721793"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-4838(97)00075-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051242397"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/bmj.38050.593634.63", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052190506"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00264a017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055167678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00429a033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055175523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00561a007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055180821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00815a004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055189340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/0264-6021:3600617", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056715897"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.172.3.1345-1351.1990", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062718704"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074889638", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075060084", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078020898", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a122349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079527700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079573520", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081436946", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082445467", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082711120", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-12", 
    "datePublishedReg": "2011-12-01", 
    "description": "Aspartate aminotransferase catalyzes the transfer of an amino group from L-aspartate to \u03b1-oxoglutarate. We have purified cytosolic isozyme, to apparent homogeneity, from the heart of river buffalo. In order to clone the enzyme total RNA was isolated and the cDNA encoding complete polypeptide of 413 amino acids was amplified by reverse transcriptase polymerase chain reaction. The cDNA and the deduced amino acid sequence exhibited 98.2% and 99.5% identities, respectively, with that of cow. The cDNA was overexpressed in Escherichia coli and the gene product was obtained in enzymologically active form. The recombinant enzyme was about 40% of the total cell proteins. The purified recombinant enzyme displayed specific activity, Km, temperature and pH stability values similar to that of the native enzyme. Edman degradation and electrospray ionization mass spectra showed that the recombinant enzyme consisted of three species having N-terminal sequences of MAPPSIF, APPSIF and PPSIF, although the second one was the predominant species. Conditions are also described, which in the mass spectral analysis give either the three species corresponding to the apo- or the holo-enzyme; the latter retaining pyridoxal phosphate bound to the protein. To our knowledge this is the first report on sequence determination, cloning and characterization of aspartate aminotransferase from river buffalo.", 
    "genre": "research_article", 
    "id": "sg:pub.10.2478/s11756-011-0125-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1017076", 
        "issn": [
          "0006-3088", 
          "1336-9563"
        ], 
        "name": "Biologia", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "66"
      }
    ], 
    "name": "First cloning and characterization of aspartate aminotransferase from river buffalo (Bubalus bubalis)", 
    "pagination": "1202", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "869b6d45ad7fb261ba1b6b7eeb0ac22ef248be7e5f3a5c1c622e54e556b3c388"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.2478/s11756-011-0125-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1028256283"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.2478/s11756-011-0125-z", 
      "https://app.dimensions.ai/details/publication/pub.1028256283"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:08", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000505.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.2478/s11756-011-0125-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.2478/s11756-011-0125-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.2478/s11756-011-0125-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.2478/s11756-011-0125-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.2478/s11756-011-0125-z'


 

This table displays all metadata directly associated to this object as RDF triples.

195 TRIPLES      21 PREDICATES      62 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.2478/s11756-011-0125-z schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Ndddd0b33f6f44ee18a7c4f9784a3343f
4 schema:citation sg:pub.10.1007/978-3-0348-9308-4_7
5 sg:pub.10.1023/a:1010618203054
6 sg:pub.10.1038/227680a0
7 sg:pub.10.1186/bcr2154
8 https://app.dimensions.ai/details/publication/pub.1074889638
9 https://app.dimensions.ai/details/publication/pub.1075060084
10 https://app.dimensions.ai/details/publication/pub.1078020898
11 https://app.dimensions.ai/details/publication/pub.1079573520
12 https://app.dimensions.ai/details/publication/pub.1081436946
13 https://app.dimensions.ai/details/publication/pub.1082445467
14 https://app.dimensions.ai/details/publication/pub.1082711120
15 https://doi.org/10.1002/9780470122778.ch2
16 https://doi.org/10.1016/0006-291x(63)90346-2
17 https://doi.org/10.1016/0009-8981(82)90071-7
18 https://doi.org/10.1016/0022-2836(84)90333-4
19 https://doi.org/10.1016/0022-2836(88)90329-4
20 https://doi.org/10.1016/0305-0491(94)00143-i
21 https://doi.org/10.1016/s0022-2836(05)80360-2
22 https://doi.org/10.1016/s0167-4838(97)00075-7
23 https://doi.org/10.1016/s1570-9639(03)00081-5
24 https://doi.org/10.1021/bi00264a017
25 https://doi.org/10.1021/bi00429a033
26 https://doi.org/10.1021/bi00561a007
27 https://doi.org/10.1021/bi00815a004
28 https://doi.org/10.1042/0264-6021:3600617
29 https://doi.org/10.1042/bj0990589
30 https://doi.org/10.1093/nar/22.22.4673
31 https://doi.org/10.1093/nar/gkq1079
32 https://doi.org/10.1093/oxfordjournals.jbchem.a122349
33 https://doi.org/10.1097/00042737-200409000-00013
34 https://doi.org/10.1104/pp.92.3.587
35 https://doi.org/10.1111/j.1432-1033.1968.tb00402.x
36 https://doi.org/10.1111/j.1432-1033.1990.tb19204.x
37 https://doi.org/10.1128/jb.172.3.1345-1351.1990
38 https://doi.org/10.1136/bmj.38050.593634.63
39 schema:datePublished 2011-12
40 schema:datePublishedReg 2011-12-01
41 schema:description Aspartate aminotransferase catalyzes the transfer of an amino group from L-aspartate to α-oxoglutarate. We have purified cytosolic isozyme, to apparent homogeneity, from the heart of river buffalo. In order to clone the enzyme total RNA was isolated and the cDNA encoding complete polypeptide of 413 amino acids was amplified by reverse transcriptase polymerase chain reaction. The cDNA and the deduced amino acid sequence exhibited 98.2% and 99.5% identities, respectively, with that of cow. The cDNA was overexpressed in Escherichia coli and the gene product was obtained in enzymologically active form. The recombinant enzyme was about 40% of the total cell proteins. The purified recombinant enzyme displayed specific activity, Km, temperature and pH stability values similar to that of the native enzyme. Edman degradation and electrospray ionization mass spectra showed that the recombinant enzyme consisted of three species having N-terminal sequences of MAPPSIF, APPSIF and PPSIF, although the second one was the predominant species. Conditions are also described, which in the mass spectral analysis give either the three species corresponding to the apo- or the holo-enzyme; the latter retaining pyridoxal phosphate bound to the protein. To our knowledge this is the first report on sequence determination, cloning and characterization of aspartate aminotransferase from river buffalo.
42 schema:genre research_article
43 schema:inLanguage en
44 schema:isAccessibleForFree true
45 schema:isPartOf N252dad784a914efb95514d06caaf3944
46 N5a2b724076f94993b6545436614d494f
47 sg:journal.1017076
48 schema:name First cloning and characterization of aspartate aminotransferase from river buffalo (Bubalus bubalis)
49 schema:pagination 1202
50 schema:productId N1fee2e52a49a40ee8bfc8a9d4772b130
51 N7502480335544af3a8eb4d99c190f72e
52 N91249547cdb249c7a29221a9b04449d8
53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028256283
54 https://doi.org/10.2478/s11756-011-0125-z
55 schema:sdDatePublished 2019-04-10T14:08
56 schema:sdLicense https://scigraph.springernature.com/explorer/license/
57 schema:sdPublisher N89ae8505628e4d919dfc66daae0df7c8
58 schema:url http://link.springer.com/10.2478/s11756-011-0125-z
59 sgo:license sg:explorer/license/
60 sgo:sdDataset articles
61 rdf:type schema:ScholarlyArticle
62 N1fee2e52a49a40ee8bfc8a9d4772b130 schema:name doi
63 schema:value 10.2478/s11756-011-0125-z
64 rdf:type schema:PropertyValue
65 N252dad784a914efb95514d06caaf3944 schema:volumeNumber 66
66 rdf:type schema:PublicationVolume
67 N53b61f83c8404dbea1adb1f9645fbe0d schema:affiliation https://www.grid.ac/institutes/grid.11173.35
68 schema:familyName Iqbal
69 schema:givenName Muzaffar
70 rdf:type schema:Person
71 N5a2b724076f94993b6545436614d494f schema:issueNumber 6
72 rdf:type schema:PublicationIssue
73 N7502480335544af3a8eb4d99c190f72e schema:name dimensions_id
74 schema:value pub.1028256283
75 rdf:type schema:PropertyValue
76 N89ae8505628e4d919dfc66daae0df7c8 schema:name Springer Nature - SN SciGraph project
77 rdf:type schema:Organization
78 N8dd83ee2f31d4648a453e231435fb89c rdf:first sg:person.01005647064.23
79 rdf:rest Nc99bbdc97fe54a78b83bfd8b97909d11
80 N91249547cdb249c7a29221a9b04449d8 schema:name readcube_id
81 schema:value 869b6d45ad7fb261ba1b6b7eeb0ac22ef248be7e5f3a5c1c622e54e556b3c388
82 rdf:type schema:PropertyValue
83 Nb74ff224ee754c51ac51ce0aee0ee5a9 rdf:first sg:person.015572356512.49
84 rdf:rest rdf:nil
85 Nc99bbdc97fe54a78b83bfd8b97909d11 rdf:first N53b61f83c8404dbea1adb1f9645fbe0d
86 rdf:rest Nd94ebbbf291e46ce8b6bf486e75d069c
87 Nd94ebbbf291e46ce8b6bf486e75d069c rdf:first sg:person.01013705746.47
88 rdf:rest Nb74ff224ee754c51ac51ce0aee0ee5a9
89 Ndddd0b33f6f44ee18a7c4f9784a3343f rdf:first sg:person.01074215514.44
90 rdf:rest N8dd83ee2f31d4648a453e231435fb89c
91 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
92 schema:name Biological Sciences
93 rdf:type schema:DefinedTerm
94 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
95 schema:name Biochemistry and Cell Biology
96 rdf:type schema:DefinedTerm
97 sg:journal.1017076 schema:issn 0006-3088
98 1336-9563
99 schema:name Biologia
100 rdf:type schema:Periodical
101 sg:person.01005647064.23 schema:affiliation https://www.grid.ac/institutes/grid.11173.35
102 schema:familyName Rashid
103 schema:givenName Naeem
104 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01005647064.23
105 rdf:type schema:Person
106 sg:person.01013705746.47 schema:affiliation https://www.grid.ac/institutes/grid.11173.35
107 schema:familyName Afza Gardner
108 schema:givenName Qurra-tul-Ann
109 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013705746.47
110 rdf:type schema:Person
111 sg:person.01074215514.44 schema:affiliation https://www.grid.ac/institutes/grid.11173.35
112 schema:familyName Shahid Nadeem
113 schema:givenName Muhammad
114 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074215514.44
115 rdf:type schema:Person
116 sg:person.015572356512.49 schema:affiliation https://www.grid.ac/institutes/grid.5491.9
117 schema:familyName Akhtar
118 schema:givenName Muhammad
119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015572356512.49
120 rdf:type schema:Person
121 sg:pub.10.1007/978-3-0348-9308-4_7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031687307
122 https://doi.org/10.1007/978-3-0348-9308-4_7
123 rdf:type schema:CreativeWork
124 sg:pub.10.1023/a:1010618203054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043560335
125 https://doi.org/10.1023/a:1010618203054
126 rdf:type schema:CreativeWork
127 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
128 https://doi.org/10.1038/227680a0
129 rdf:type schema:CreativeWork
130 sg:pub.10.1186/bcr2154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005518856
131 https://doi.org/10.1186/bcr2154
132 rdf:type schema:CreativeWork
133 https://app.dimensions.ai/details/publication/pub.1074889638 schema:CreativeWork
134 https://app.dimensions.ai/details/publication/pub.1075060084 schema:CreativeWork
135 https://app.dimensions.ai/details/publication/pub.1078020898 schema:CreativeWork
136 https://app.dimensions.ai/details/publication/pub.1079573520 schema:CreativeWork
137 https://app.dimensions.ai/details/publication/pub.1081436946 schema:CreativeWork
138 https://app.dimensions.ai/details/publication/pub.1082445467 schema:CreativeWork
139 https://app.dimensions.ai/details/publication/pub.1082711120 schema:CreativeWork
140 https://doi.org/10.1002/9780470122778.ch2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024891027
141 rdf:type schema:CreativeWork
142 https://doi.org/10.1016/0006-291x(63)90346-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041824850
143 rdf:type schema:CreativeWork
144 https://doi.org/10.1016/0009-8981(82)90071-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047848829
145 rdf:type schema:CreativeWork
146 https://doi.org/10.1016/0022-2836(84)90333-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033880362
147 rdf:type schema:CreativeWork
148 https://doi.org/10.1016/0022-2836(88)90329-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037885513
149 rdf:type schema:CreativeWork
150 https://doi.org/10.1016/0305-0491(94)00143-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1007482456
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1016/s0022-2836(05)80360-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013618994
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1016/s0167-4838(97)00075-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051242397
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1016/s1570-9639(03)00081-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033423229
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1021/bi00264a017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055167678
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1021/bi00429a033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055175523
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1021/bi00561a007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055180821
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1021/bi00815a004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055189340
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1042/0264-6021:3600617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056715897
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1042/bj0990589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009462695
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1093/nar/22.22.4673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042438223
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1093/nar/gkq1079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038685652
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1093/oxfordjournals.jbchem.a122349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079527700
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1097/00042737-200409000-00013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010207753
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1104/pp.92.3.587 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050721793
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1111/j.1432-1033.1968.tb00402.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1021767698
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1111/j.1432-1033.1990.tb19204.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002959078
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1128/jb.172.3.1345-1351.1990 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062718704
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1136/bmj.38050.593634.63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052190506
187 rdf:type schema:CreativeWork
188 https://www.grid.ac/institutes/grid.11173.35 schema:alternateName University of the Punjab
189 schema:name Calzyme Laboratories, Inc., 3443 Miguelito Court, 93401, San Luis Obispo, CA, USA
190 School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan
191 rdf:type schema:Organization
192 https://www.grid.ac/institutes/grid.5491.9 schema:alternateName University of Southampton
193 schema:name School of Biological Sciences, University of Southampton, SO16 7PX, Southampton, UK
194 School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan
195 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...