Gene cloning and characterization of glycine oxidase from newly isolated Bacillus subtilis strain R5 View Full Text


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Article Info

DATE

2011-02

AUTHORS

Farrukh Jamil, Naeem Rashid, Qurra-tul-Ann Afza Gardner, Muhammad Akhtar

ABSTRACT

The gene encoding the glycine oxidase from Bacillus subtilis strain R5 (goxR) was cloned and expressed in Escherichia coli. The gene consisted of 1,110 nucleotides that encoded a protein (GoxR) of 369 amino acid residues with a molecular mass of 40,761 Da. The GoxR exhibited 98.6% identity with glycine oxidase from B. subtilis strain 168. Gene expression and purification of the recombinant GoxR were performed. The recombinant GoxR existed in a homotetramer form. The recombinant protein effectively catalyzed the oxidation of glycine and d-alanine. The specific activity of the purified recombinant GoxR was 0.96 U/mg when glycine was used as a substrate and 1.0 U/mg when d-alanine was substrate. The enzyme displayed its highest activity at pH 8.0 and at a temperature of 50°C. The activation energy of the reaction catalyzed by the enzyme was calculated to be 26 kJ/mol. The enzyme activity was significantly inhibited in the presence of organic solvents. No enhancement of enzyme activity was observed in the presence of metal cations. The experimental results presented in this study demonstrate that the enzyme was a bonafide glycine oxidase. More... »

PAGES

1-7

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http://scigraph.springernature.com/pub.10.2478/s11756-010-0141-4

DOI

http://dx.doi.org/10.2478/s11756-010-0141-4

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