Epidemiological survey of severe fever with thrombocytopenia syndrome virus in ticks in Nagasaki, Japan View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015

AUTHORS

Daisuke Hayasaka, Satoshi Shimada, Kotaro Aoki, Yuki Takamatsu, Leo Uchida, Masahiro Horio, Yu Fuxun, Kouichi Morita

ABSTRACT

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease endemic in East Asia. Transmitted to other organisms by infected ticks, the SFTS virus (SFTSV) and is endemic to Nagasaki in western Japan. However, epidemiological information regarding SFTSV in Nagasaki ticks has not been available to date. In this study, we began by examining the sensitivities of SFTSV gene detection by real-time RT-PCR and virus isolation in cultured cells and mice. These methods could detect SFTSV in the samples containing more than 4 × 10(0) ffu. Next, we attempted to isolate SFTSV and to detect viral gene in 2,222 nymph and adult ticks collected from May to August 2013 among seven regions of Nagasaki. However, neither virus isolation nor viral gene detection were confirmed in the tick pools. SFTSV positivity rates are considered to be very low in ticks, and viral loads are also very limited. Further investigations increasing the number of ticks and including larval samples as well as improved detection methods, may be required to find SFTSV-positive ticks in this region. More... »

PAGES

159-164

Identifiers

URI

http://scigraph.springernature.com/pub.10.2149/tmh.2015-01

DOI

http://dx.doi.org/10.2149/tmh.2015-01

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050322679

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26543390


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