Microfluorometric assay for quantification of anti-erythrocytic antibody level in sera of Plasmodium berghei ANKA infected semi-immune mice View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010

AUTHORS

Gideon K Helegbe, Nguyen T Huy, Tetsuo Yanagi, Mohammed N Shuaibu, Mihoko Kikuchi, Michio Yasunami, Kenji Hirayama

ABSTRACT

A simple, sensitive and reproducible fluorometry method was developed to quantify anti-erythrocytic auto-antibody in the sera of semi-immune mice. The level of anti-erythrocytic auto-antibody measured using fluorometry was significantly higher in sera of Plasmodium berghei ANKA infected mice than the uninfected, p‹0.0001. This result correlates significantly with the standard ELISA assay, p‹0.0001, r2=0.7766. Both methods show significant correlation with regard to extent of Hb loss, p‹0.006 (r2=0.33) (fluorometry method) and p‹0.0001, r2=0.30 (ELISA). Thus the fluorometry method may serve as an alternative to standard ELISA assay and can be modified to suit similar objectives. More... »

PAGES

127-131

Identifiers

URI

http://scigraph.springernature.com/pub.10.2149/tmh.2010-13

DOI

http://dx.doi.org/10.2149/tmh.2010-13

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023357127


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