Molecular Beacon-Based Homogeneous Fluorescence PCR Assay for the Diagnosis of Infectious Diseases View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2000-02-03

AUTHORS

Qing-Ge Li, Ji-Xuan Liang, Guo-Yan Luan, Yang Zhang, Kun Wang

ABSTRACT

A rapid and simple homogeneous fluorescence PCR assay was developed for the clinical diagnosis of infectious diseases based on a molecular beacon. The established method could reproducibly detect Mycobacterium tuberculosis at the 10 bacteria/mL level. The analytical specificity was tested with 14 strains of mycobacterium, four unrelated bacteria and 220 negative samples; no false positive results were obtained. A blind test was also performed to evaluate its performance in Mycobacterium tuberculosis diagnosis. The results showed that both the clinical sensitivity and the specificity were 100%, and that the detection limit was in the range of 1–10 bacteria/ml. A clinical study with 466 patient samples demonstrated that fluorescence PCR assay correlated well with smear (93.6%) and culture (98.4%) methods for positive samples. However, fluorescence PCR could detect positive samples (62.9%) more than smear (30.3%) and culture (31.4%), indicating a higher sensitivity of the present method than the traditional ones. The feasibility of this method was further approved by successful detection of Neisseria gonorrhoeae and Chlamydia trachomatis. More... »

PAGES

245-248

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.2116/analsci.16.245

DOI

http://dx.doi.org/10.2116/analsci.16.245

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046656396


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