Isolation and HPTLC Analysis of Iridoids in Premna integrifolia, an Important Ingredient of Ayurvedic Drug Dashmool View Full Text


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Article Info

DATE

2013-06-01

AUTHORS

Deepti Yadav, Madan Mohan Gupta

ABSTRACT

Premna integrifolia is an important constituent of the famous herbal formulation “Dashmool” of the Indian Ayurvedic system of medicines. The plant is traditionally used in the treatment of skin diseases, arthritis, gonorrhea, rheumatism, anorexia, and jaundice. 10-O-trans-p-coumaroylcatalpol (A), 4″-hydroxy-E-globularinin (B), and premnosidic acid (C) are major iridoid glycosides (chemical markers) present in P. integrifolia. In the present study, an attempt has been made to develop a high-performance thin-layer chromatography (HPTLC) method for quantitative estimation of iridoid glycosides in the stem bark of P. integrifolia. Separation was performed on silica gel 60F254 high-performance thin-layer chromatographic plates. The solvent system consisted of ethyl acetate-methanol-H2 O-acetic acid (80:12:6:2 v/v). Densitometric analysis of iridoids was carried out in the absorbance mode at 510 nm after post-chromatographic derivatization using vanillin-sulphuric acid reagent. The method was validated for peak purity, precision, robustness, limit of detection (LOD), and quantitation (LOQ) etc., as per the International Conference on Harmonization (ICH) guidelines. This HPTLC method was found to be reproducible, accurate, and can detect iridoids at a nanogram level. The developed HPTLC method would be an important tool in the quality control method for polyherbal formulations. More... »

PAGES

260-266

Identifiers

URI

http://scigraph.springernature.com/pub.10.1556/jpc.26.2013.3.10

DOI

http://dx.doi.org/10.1556/jpc.26.2013.3.10

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1067888488


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