Use of HPTLC, HPLC, and Densitometry for Qualitative Separation of Indole Alkaloids from Rauvolfia serpentina Roots View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-08-01

AUTHORS

Madan Mohan Gupta, Alpana Srivastava, Arvind Kumar Tripathi, Himanshu Misra, Ram Kishore Verma

ABSTRACT

High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids. More... »

PAGES

282-287

Identifiers

URI

http://scigraph.springernature.com/pub.10.1556/jpc.19.2006.4.5

DOI

http://dx.doi.org/10.1556/jpc.19.2006.4.5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1067887889


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0301", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Analytical Chemistry", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gupta", 
        "givenName": "Madan Mohan", 
        "id": "sg:person.01242460441.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Srivastava", 
        "givenName": "Alpana", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tripathi", 
        "givenName": "Arvind Kumar", 
        "id": "sg:person.01044210077.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044210077.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Misra", 
        "givenName": "Himanshu", 
        "id": "sg:person.01001067434.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001067434.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Verma", 
        "givenName": "Ram Kishore", 
        "id": "sg:person.01212430255.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212430255.34"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2006-08-01", 
    "datePublishedReg": "2006-08-01", 
    "description": "High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids.", 
    "genre": "article", 
    "id": "sg:pub.10.1556/jpc.19.2006.4.5", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1099646", 
        "issn": [
          "0933-4173", 
          "1789-0993"
        ], 
        "name": "JPC \u2013 Journal of Planar Chromatography \u2013 Modern TLC", 
        "publisher": "Akademiai Kiado Zrt.", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "keywords": [
      "efficient extraction", 
      "normal-phase separation", 
      "separation", 
      "reversed-phase separation", 
      "image analysis", 
      "qualitative separation", 
      "plate", 
      "extraction", 
      "hexane", 
      "high-performance thin-layer chromatography", 
      "use of chloroform", 
      "HPTLC plates", 
      "technique", 
      "use", 
      "chloroform", 
      "methanol", 
      "components", 
      "high performance liquid chromatography", 
      "results", 
      "different chromatograms", 
      "loss", 
      "analysis", 
      "performance liquid chromatography", 
      "presence", 
      "roots", 
      "water extract", 
      "liquid chromatography", 
      "chromatography", 
      "chromatograms", 
      "thin-layer chromatography", 
      "densitometry", 
      "extracts", 
      "alkaloids", 
      "computerized densitometry", 
      "root extract", 
      "indole alkaloids", 
      "ajmalicine", 
      "serpentina roots", 
      "ajmaline", 
      "components of hexane", 
      "Rauvolfia serpentina roots", 
      "two-dimensional spectrographic image analysis", 
      "spectrographic image analysis", 
      "serpentina root extracts", 
      "marker indole alkaloids", 
      "most efficient extraction", 
      "Use of HPTLC"
    ], 
    "name": "Use of HPTLC, HPLC, and Densitometry for Qualitative Separation of Indole Alkaloids from Rauvolfia serpentina Roots", 
    "pagination": "282-287", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1067887889"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1556/jpc.19.2006.4.5"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1556/jpc.19.2006.4.5", 
      "https://app.dimensions.ai/details/publication/pub.1067887889"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1556/jpc.19.2006.4.5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1556/jpc.19.2006.4.5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1556/jpc.19.2006.4.5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1556/jpc.19.2006.4.5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1556/jpc.19.2006.4.5'


 

This table displays all metadata directly associated to this object as RDF triples.

132 TRIPLES      21 PREDICATES      72 URIs      64 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1556/jpc.19.2006.4.5 schema:about anzsrc-for:03
2 anzsrc-for:0301
3 schema:author Nea27bb3a45aa42d389d5e918c0785e4d
4 schema:datePublished 2006-08-01
5 schema:datePublishedReg 2006-08-01
6 schema:description High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids.
7 schema:genre article
8 schema:inLanguage en
9 schema:isAccessibleForFree false
10 schema:isPartOf N3984539fa3724e2282fd21ffbabf3870
11 N9ea5701ade0e43988e5fd9843236657c
12 sg:journal.1099646
13 schema:keywords HPTLC plates
14 Rauvolfia serpentina roots
15 Use of HPTLC
16 ajmalicine
17 ajmaline
18 alkaloids
19 analysis
20 chloroform
21 chromatograms
22 chromatography
23 components
24 components of hexane
25 computerized densitometry
26 densitometry
27 different chromatograms
28 efficient extraction
29 extraction
30 extracts
31 hexane
32 high performance liquid chromatography
33 high-performance thin-layer chromatography
34 image analysis
35 indole alkaloids
36 liquid chromatography
37 loss
38 marker indole alkaloids
39 methanol
40 most efficient extraction
41 normal-phase separation
42 performance liquid chromatography
43 plate
44 presence
45 qualitative separation
46 results
47 reversed-phase separation
48 root extract
49 roots
50 separation
51 serpentina root extracts
52 serpentina roots
53 spectrographic image analysis
54 technique
55 thin-layer chromatography
56 two-dimensional spectrographic image analysis
57 use
58 use of chloroform
59 water extract
60 schema:name Use of HPTLC, HPLC, and Densitometry for Qualitative Separation of Indole Alkaloids from Rauvolfia serpentina Roots
61 schema:pagination 282-287
62 schema:productId N7e6b0869888c4d97856a2a1baa1ab4c0
63 N93080df40f0348c1acd4827f5ca4e744
64 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067887889
65 https://doi.org/10.1556/jpc.19.2006.4.5
66 schema:sdDatePublished 2022-01-01T18:15
67 schema:sdLicense https://scigraph.springernature.com/explorer/license/
68 schema:sdPublisher Nf04f0dfc5b214d3f8641abebe5b54ac1
69 schema:url https://doi.org/10.1556/jpc.19.2006.4.5
70 sgo:license sg:explorer/license/
71 sgo:sdDataset articles
72 rdf:type schema:ScholarlyArticle
73 N3984539fa3724e2282fd21ffbabf3870 schema:volumeNumber 19
74 rdf:type schema:PublicationVolume
75 N528509af72684608864dd94bd2ac2984 rdf:first sg:person.01044210077.92
76 rdf:rest Nbb50e376fb7f480e8df43381aced411a
77 N60f78abc12684514b08947adb979f8d5 rdf:first sg:person.01212430255.34
78 rdf:rest rdf:nil
79 N754e5bc2c90a4ae0bd761476f1a6b9d4 rdf:first N8e2ed225e7b64f9ca038f89ff6e49cb4
80 rdf:rest N528509af72684608864dd94bd2ac2984
81 N7e6b0869888c4d97856a2a1baa1ab4c0 schema:name dimensions_id
82 schema:value pub.1067887889
83 rdf:type schema:PropertyValue
84 N8e2ed225e7b64f9ca038f89ff6e49cb4 schema:affiliation grid-institutes:grid.417631.6
85 schema:familyName Srivastava
86 schema:givenName Alpana
87 rdf:type schema:Person
88 N93080df40f0348c1acd4827f5ca4e744 schema:name doi
89 schema:value 10.1556/jpc.19.2006.4.5
90 rdf:type schema:PropertyValue
91 N9ea5701ade0e43988e5fd9843236657c schema:issueNumber 4
92 rdf:type schema:PublicationIssue
93 Nbb50e376fb7f480e8df43381aced411a rdf:first sg:person.01001067434.22
94 rdf:rest N60f78abc12684514b08947adb979f8d5
95 Nea27bb3a45aa42d389d5e918c0785e4d rdf:first sg:person.01242460441.07
96 rdf:rest N754e5bc2c90a4ae0bd761476f1a6b9d4
97 Nf04f0dfc5b214d3f8641abebe5b54ac1 schema:name Springer Nature - SN SciGraph project
98 rdf:type schema:Organization
99 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
100 schema:name Chemical Sciences
101 rdf:type schema:DefinedTerm
102 anzsrc-for:0301 schema:inDefinedTermSet anzsrc-for:
103 schema:name Analytical Chemistry
104 rdf:type schema:DefinedTerm
105 sg:journal.1099646 schema:issn 0933-4173
106 1789-0993
107 schema:name JPC – Journal of Planar Chromatography – Modern TLC
108 schema:publisher Akademiai Kiado Zrt.
109 rdf:type schema:Periodical
110 sg:person.01001067434.22 schema:affiliation grid-institutes:grid.417631.6
111 schema:familyName Misra
112 schema:givenName Himanshu
113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001067434.22
114 rdf:type schema:Person
115 sg:person.01044210077.92 schema:affiliation grid-institutes:grid.417631.6
116 schema:familyName Tripathi
117 schema:givenName Arvind Kumar
118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044210077.92
119 rdf:type schema:Person
120 sg:person.01212430255.34 schema:affiliation grid-institutes:grid.417631.6
121 schema:familyName Verma
122 schema:givenName Ram Kishore
123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212430255.34
124 rdf:type schema:Person
125 sg:person.01242460441.07 schema:affiliation grid-institutes:grid.417631.6
126 schema:familyName Gupta
127 schema:givenName Madan Mohan
128 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07
129 rdf:type schema:Person
130 grid-institutes:grid.417631.6 schema:alternateName Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India
131 schema:name Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, 226015, Lucknow, India
132 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...