Immobilisation of Aspergillus oryzae α-amylase and Aspergillus niger glucoamylase enzymes as cross-linked enzyme aggregates View Full Text


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Article Info

DATE

2014-12-30

AUTHORS

A. Sercan Sahutoglu, Cahit Akgul

ABSTRACT

Cross-linked enzyme aggregates (CLEA) of Aspergillus oryzea α-amylase (AoAA) and Aspergillus niger glucoamylase (AnGA) were prepared using glutaraldehyde and dextran polyaldehyde as crosslinkers. The maximum activity recoveries for glutaraldehyde cross-linking were 21.8 % and 41.2 %, respectively. The addition of a proteic feeder (bovine serum albumin) exhibited a negative effect on the activity recoveries for both enzymes. Dextran polyaldehyde was used as a cross-linking agent instead of glutaraldehyde to reduce the activity losses. As a result, an activity recovery of 60.0 % was obtained for Aspergillus oryzea α-amylase. On the other hand, no activity recovery was observed for Aspergillus niger glucoamylase due to the latter enzyme’s affinity for dextran. More... »

PAGES

433-439

Identifiers

URI

http://scigraph.springernature.com/pub.10.1515/chempap-2015-0031

DOI

http://dx.doi.org/10.1515/chempap-2015-0031

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1067527393


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