Principles and practices of laser scanning confocal microscopy View Full Text


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Article Info

DATE

2000-10

AUTHORS

Stephen W. Paddock

ABSTRACT

The laser scanning confocal microscope (LSCM) is an essential tool for many biomedical imaging applications at the level of the light microscope. The basic principles of confocal microscopy and the evolution of the LSCM into today's sophisticated instruments are outlined. The major imaging modes of the LSCM are introduced including single optical sections, multiple wavelength images, three-dimensional reconstructions, and living cell and tissue sequences. Practical aspects of specimen preparation, image collection, and image presentation are included along with a primer on troubleshooting the LSCM for the novice. More... »

PAGES

127-149

References to SciGraph publications

  • 1995. Visualization Systems for Multidimensional CLSM Images in HANDBOOK OF BIOLOGICAL CONFOCAL MICROSCOPY
  • 1995. Objective Lenses for Confocal Microscopy in HANDBOOK OF BIOLOGICAL CONFOCAL MICROSCOPY
  • 1995. The Role of the Pinhole in Confocal Imaging System in HANDBOOK OF BIOLOGICAL CONFOCAL MICROSCOPY
  • 1998-12-07. Information Management of Confocal Microscopy Images: Traditional Text-Based Databases and Image Gallery Databases in CONFOCAL MICROSCOPY
  • 1996-12. Use of a cDNA microarray to analyse gene expression patterns in human cancer in NATURE GENETICS
  • 1995. Confocal Microscopy of Living Cells in HANDBOOK OF BIOLOGICAL CONFOCAL MICROSCOPY
  • 1998-12-07. Imaging Gene Expression Using Antibody Probes in CONFOCAL MICROSCOPY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1385/mb:16:2:127

    DOI

    http://dx.doi.org/10.1385/mb:16:2:127

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1020007713

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/11131973


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