Pretreatment with ammonia water for enzymatic hydrolysis of corn husk, bagasse, and switchgrass View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-03

AUTHORS

Masahiro Kurakake, Wataru Kisaka, Kazuya Ouchi, Toshiaki Komaki

ABSTRACT

Bagasse, corn husk, and switchgrass were pretreated with ammonia water to enhance enzymatic hydrolysis. The sample (2 g) was mixed with 1-6 mL ammonia water (25-28% ammonia) and autoclaved at 120 degreesC for 20 min. After treatment, the product was vacuum-dried to remove ammonia gas. The dried solid could be used immediately in the enzymatic hydrolysis without washing. The enzymatic hydrolysis was effectively improved with more than 0.5 and 1 mL ammonia water/g for corn husk and bagasse, respectively. In bagasse, glucose, xylose, and xylobiose were the main products. The adsorption of CMCase and xylanase was related to the initial rate of enzymatic hydrolysis. In corn husks, arabinoxylan extracted by pretreatment was substantially unhydrolyzed because of the high ratio of arabinose to xylose (0.6). The carbohydrate yields from cellulose and hemicellulose were 72.9% and 82.4% in bagasse, and 86.2% and 91.9% in corn husk, respectively. The ammonia/water pretreatment also benefited from switchgrass (Miscanthus sinensis and Solidago altissima L.) hydrolysis. More... »

PAGES

251-259

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/abab:90:3:251

DOI

http://dx.doi.org/10.1385/abab:90:3:251

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020743532

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11318037


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