Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosic biomass View Full Text


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Article Info

DATE

2000-03

AUTHORS

Bruce S. Dien, Nancy N. Nichols, Patricia J. O'Bryan, Rodney J. Bothast

ABSTRACT

Two new ethanologenic strains (FBR4 and FBR5) of Escherichia coli were constructed and used to ferment corn fiber hydrolysate. The strains carry the plasmid pLO1297, which contains the genes from Zymomonas mobilis necessary for efficiently converting pyruvate into ethanol. Both strains selectively maintained the plasmid when grown anaerobically. Each culture was serially transferred 10 times in anaerobic culture with sugar-limited medium containing xylose, but noselective antibiotic. An average of 93 and 95% of the FBR4 and FBR5 cells, respectively, maintained pLO1297 in anaerobic culture. The fermentation performances of the repeatedly transferred cultures were compared with those of cultures freshly revived from stock in pH-controlled batch fermentations with 10% (w/v) xylose. Fermentation results were similar for all the cultures. Fermentations were completed within 60 h and ethanol yields were 86–92% of theoretical. Maximal ethanol concentrations were 3.9–4.2% (w/v). The strains were also tested for their ability to ferment corn fiber hydrolysate, which contained 8.5% (w/v) total sugars (2.0% arabinose, 2.8% glucose, and 3.7% xylose). E. coli FBR5 produced more ethanol than FBR4 from the corn fiber hydrolysate. E. coli FBR5 fermented all but 0.4% (w/v) of the available sugar, whereas strain FBR4 left 1.6% unconsumed. The fermentation with FBR5 was completed within 55 h and yielded 0.46 g of ethanol/g of available sugar, 90% of the maximum obtainable. More... »

PAGES

181-196

References to SciGraph publications

  • 1999-06. Conversion of corn fiber to ethanol by recombinant E. coli strain FBR3 in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 1992-09. Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinantEscherichia coli B containing integrated genes for ethanol production in BIOTECHNOLOGY LETTERS
  • 1996-03. The relationship between growth enhancement andpet expression inEscherichia coli in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1997-05. Fermentation of corn fibre sugars by an engineered xylose utilizing Saccharomyces yeast strain in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 1996-09. Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast in BIOTECHNOLOGY LETTERS
  • 1997-11. Conversion of corn milling fibrous co-products into ethanol by recombinant Escherichia coli strains K011 and SL40 in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 1998-03. Cofermentation of glucose, xylose, and arabinose by mixed cultures of two genetically engineeredZymomonas mobilis strains in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1998-03. Continuous culture studies of xylose-fermentingZymomonas mobilis in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1995-07. Loss of ethanologenicity in Escherichia coli B recombinants pLOI297 and KO11 during growth in the absence of antibiotics in BIOTECHNOLOGY LETTERS
  • 1997-03. Enhanced cofermentation of glucose and xylose by recombinantSaccharomyces yeast strains in batch and continuous operating modes in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1998-03. A novel fermentation pathway in anEscherichia coli mutant producing succinic acid, acetic acid, and ethanol in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1996-01. Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineeredEscherichia coli strain KO11 in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1385/abab:84-86:1-9:181

    DOI

    http://dx.doi.org/10.1385/abab:84-86:1-9:181

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1040602350

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10849788


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