<i>Agrobacterium</i> Virulence Gene Induction View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2006-06-01

AUTHORS

Wang Kan , Stanton B. Gelvin

ABSTRACT

The ability of Agrobacterium to transform plants and other organisms is under highly regulated genetic control. Two Virulence (Vir) proteins, VirA and VirG, function as a two-component regulatory system to sense particular phenolic compounds synthesized by wounded plant tissues. Induction by these phenolic compounds, in the presence of certain neutral or acid sugars, results in activation of other vir genes, leading to the processing of T-DNA from the Ti-plasmid and transfer of T-DNA to recipient host cells. Many plant, and most nonplant, species do not provide sufficient quantities of the correct phenolic compounds to permit efficient Agrobacterium-mediated genetic transformation to occur. In order to transform these species, phenolic inducing compounds must be added to agrobacteria before and/or during cocultivation of recipient cells with the bacteria. This chapter discusses conditions for efficient induction of Agrobacterium virulence genes by phenolic compounds. More... »

PAGES

77-84

Book

TITLE

Agrobacterium Protocols

ISBN

1-59745-130-4

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59745-130-4:77

DOI

http://dx.doi.org/10.1385/1-59745-130-4:77

DIMENSIONS

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