Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2005-11-01

AUTHORS

Krit Raemakers , Isolde Pereira , Herma Koehorst van Putten , Richard Visser

ABSTRACT

In cassava both direct and indirect somatic embryogenesis is described. Direct somatic embryogenesis starts with the culture of leaf explants on Murashige and Skoog (MS) medium supplemented with auxins. Somatic embryos undergo secondary somatic embryogenesis when cultured on the same medium.Indirect somatic embryogenesis is initiated by subculture of directly induced embryogenic tissue on auxin-supplemented medium with Gresshoff and Doy salts and vitamins. A very fine friable embryogenic callus (FEC) is formed after a few rounds of subculture and stringent selection. This FEC is maintained by subculture on auxin supplemented medium. Lowering of the auxin concentration allows the FEC to form mature somatic embryos that develop into plants when transferred to a cytokinin-supplemented medium. More... »

PAGES

101-109

Book

TITLE

Plant Cell Culture Protocols

ISBN

978-1-58829-547-7
978-1-59259-959-2

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-959-1:101

DOI

http://dx.doi.org/10.1385/1-59259-959-1:101

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032161412


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