Interaction of p53 with Cellular Proteins View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2003-06-20

AUTHORS

Sumitra Deb , Swati Palit Deb , David M. Keller , Shelya X. Zeng , Hua Lu

ABSTRACT

Cellular proteins that interact with p53 play a major role in both positive and negative regulation of this tumor suppressor and can fine-tune its response to specific cellular stresses. As a consequence, p53 biology will not be complete until these interacting proteins are fully characterized. This chapter outlines two methods for identifying and characterizing p53-binding proteins: (i) glutathione-S-transferase (GST) protein-protein interaction assay; and (ii) co-immunoprecipitation (co-IP) assay. These two methods are ideal for any laboratory to perform; the assays are short in duration, do not require specialized expertise to establish in the laboratory, give reliable and reproducible data, and are cost-efficient, because few reagents are needed. This chapter gives a basic description of these two techniques and provides tips that are not found in other protocol manuals on how to achieve the best results. More... »

PAGES

121-134

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-408-5:121

DOI

http://dx.doi.org/10.1385/1-59259-408-5:121

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038790097

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12824529


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1385/1-59259-408-5:121'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1385/1-59259-408-5:121'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1385/1-59259-408-5:121'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1385/1-59259-408-5:121'


 

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