Reduction of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences from Modern and Ancient DNA View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2003-08-01

AUTHORS

John M.S. Bartlett , David Stirling , Wera M. Schmerer

ABSTRACT

Repetitive sequences like short tandem repeat (STR) loci are generally referred to as slippery DNA (1). They owe this nickname to a characteristic leading to slippage within the primer-template complex during PCR elongation of the new strand (2,3), resulting in the synthesis of byproducts shortened by one repeat unit compared with the original sequence. The generation of these so-called shadow bands (4) is a well-known problem connected with the amplification of repetitive DNA, complicating the genotype analysis of modern (e.g., ref. 5), forensic (6), and ancient (7,8) specimens. In some applications, the occurrence of this artifact makes it necessary to develop guidelines for allele designation (6,9). More... »

PAGES

309-314

Book

TITLE

PCR Protocols

ISBN

1-59259-384-4

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-384-4:309

DOI

http://dx.doi.org/10.1385/1-59259-384-4:309

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047244826


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