cDNA Generation on Paramagnetic Beads View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2003-02-19

AUTHORS

Shao-Yao Ying , Zhaohui Wang , Michael G. K. Jones

ABSTRACT

Synthesis of complementary DNA (cDNA) by reverse transcription (RT) is a key step in investigating specific gene expression of a single transcript by RT-PCR (polymerase chain reaction) or to study the more complex profiles of gene expression in a biological sample using cDNA library or other techniques. Solid-phase generation of cDNA (1), based on immobilization of the synthesized cDNA on paramagnetic beads and the magnetic-bead separation technology, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (see Note 1). Using this method, analysis of gene expression can be carried out at a single-cell level (2–4). This is not possible using conventional techniques of mRNA isolation and cDNA cloning. More... »

PAGES

25-32

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1385/1-59259-359-3:25

DOI

http://dx.doi.org/10.1385/1-59259-359-3:25

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047313246

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12703730


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